SONG PRESENTATION INDUCES GENE-EXPRESSION IN THE SONGBIRD FOREBRAIN
SONG PRESENTATION INDUCES GENE-EXPRESSION IN THE SONGBIRD FOREBRAIN
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DOI：
10.1073/pnas.89.15.6818
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发表时间：
1992-08-01
影响因子：
11.1
通讯作者：
CLAYTON, DF
中科院分区：
文献类型：
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作者：
MELLO, CV;VICARIO, DS;CLAYTON, DF
我们研究了基因组调控事件的参与，在鸣禽大脑的反应，已知的生理和行为相关的自然听觉刺激，鸟鸣。使用原位杂交，我们检测到一个即时早期基因编码的转录调节因子（ZENK;也被称为zif-268，ZENK-1，NGFI-A，或Krox-24）的前脑mRNA水平的快速增加后，介绍磁带记录歌曲的金丝雀（Serinus canaria）和斑胸草雀（Taeniopygia guttata）。ZENK感应在被认为参与听觉处理的前脑区域最明显，当鸟类听到自己物种的歌声时，ZENK感应最大。当鸟类听到不同物种的歌声时，诱导水平显着降低，并且在暴露于短纯音后看不到诱导。细胞分析表明，诱导的水平反映了被募集来表达基因的神经元的比例。这些结果表明，基因组反应在与歌曲模式识别、辨别或听觉关联形成相关的神经过程中发挥作用。
We investigated the participation of genomic regulatory events in the response of the songbird brain to a natural auditory stimulus of known physiological and behavioral relevance, birdsong. Using in situ hybridization, we detected a rapid increase in forebrain mRNA levels of an immediate-early gene encoding a transcriptional regulator (ZENK; also known as zif-268, egr-1, NGFI-A, or Krox-24) following presentation of tape-recorded songs to canaries (Serinus canaria) and zebra finches (Taeniopygia guttata). ZENK induction is most marked in a forebrain region believed to participate in auditory processing and is greatest when birds hear the song of their own species. A significantly lower level of induction occurs when birds hear the song of a different species and no induction is seen after exposure to tone bursts. Cellular analysis indicates that the level of induction reflects the proportion of neurons recruited to express the gene. These results suggest a role for genomic responses in neural processes linked to song pattern recognition, discrimination, or the formation of auditory associations.