REORGANIZATION OF CYTOSKELETAL AND JUNCTIONAL PROTEINS DURING COCHLEAR HAIR CELL DEGENERATION
REORGANIZATION OF CYTOSKELETAL AND JUNCTIONAL PROTEINS DURING COCHLEAR HAIR CELL DEGENERATION
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DOI：
10.1002/cm.970180307
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发表时间：
1991-01-01
影响因子：
--
通讯作者：
ALTSCHULER, RA
中科院分区：
文献类型：
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作者：
RAPHAEL, Y;ALTSCHULER, RA
实验旨在阐明当毛细胞退化并形成指骨疤痕时，Corti器官中细胞骨架元件和细胞间连接的变化。将豚鼠暴露于高强度噪声中，可诱导毛细胞损伤。利用扫描电镜、透射电镜和组织化学研究了微丝、中间丝和紧密连接特异性蛋白组织的时空变化。结果表明，微丝、细胞角蛋白、粘附连接和紧密连接在损伤区域重新排列其分布。从这些变化的时间序列来看，指骨疤痕似乎与毛细胞变性同时发生，并且Corti器官腔膜的完整性没有中断。每个疤痕是由两个支持细胞形成的，它们扩展并侵入垂死毛细胞的亚根尖区域。这个区域变成细胞角蛋白阳性。两个支持细胞在疤痕的中线相遇，在那里形成了一个新的连接复合物。连接复合体由紧密连接和粘附型连接组成，但没有桥粒。
Experiments were carried out to elucidate changes in cytoskeletal elements and intercellular junctions in the organ of Corti, when hair cells degenerate and phalangeal scars form. Hair cell damage was induced by exposing guinea pigs to high intensity noise. The spatial and temporal changes in the organization of microfilaments, intermediate filaments, and tight junction-specific proteins were investigated using scanning and transmission electron microscopy and histochemistry. The results show that microfilaments, cytokeratins, adherens junctions, and tight junctions rearrange their distribution in damaged areas. From the temporal sequence of these changes it appears that phalangeal scars develop simultaneous with hair cell degeneration, and that the integrity of the luminal membranes in the organ of Corti is not interrupted. Each scar is formed by two supporting cells which expand and invade the sub-apical region of the dying hair cell. This region becomes cytokeratin-positive. The two supporting cells meet at the mid-line of the scar, where a new junctional complex is formed. The junctional complex consists of tight junction and adherens-type junction, but desmosomes are absent.