Using 915 nm Laser Excited Tm3+/Er3+/Ho3+-Doped NaYbF4 Upconversion Nanoparticles for in Vitro and Deeper in Vivo Bioimaging without Overheating Irradiation
Using 915 nm Laser Excited Tm3+/Er3+/Ho3+-Doped NaYbF4 Upconversion Nanoparticles for in Vitro and Deeper in Vivo Bioimaging without Overheating Irradiation
复制标题
使用 915 nm 激光激发 Tm3 /Er3 /Ho3 掺杂 NaYbF4 上转换纳米颗粒进行体外和更深体内生物成像，无需过热照射
DOI：
10.1021/nn200110j
复制
发表时间：
2011-05-01
期刊：
影响因子：
17.1
通讯作者：
Andersson-Engels, Stefan
中科院分区：
文献类型：
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作者：
Zhan, Qiuqiang;Qian, Jun;Andersson-Engels, Stefan
超高对比度上转换（UC）生物成像的成功进一步发展需要解决现有的悖论：980 nm激光用于激发上转换纳米粒子（UCNPs），而980 nm光对水和生物标本具有强的光吸收。本文首次对980 nm激发激光在UC生物成像中引起的过热问题进行了计算和实验研究。提出了一种新的有前途的激发方法，用于更好的近红外到近红外（nir到nir） UC光致发光体外或体内成像。这种新颖的激光激发方法提供了大大减少加热的生物标本和更大的成像深度在动物或组织由于相当低的吸水性。实验获得了小鼠在激光加热下的热成像图，以证明915 nm激光的低加热优势。我们的组织模体实验和模拟验证了915 nm激光在深层组织成像方面优于980 nm激光。一种新颖而简单的表面功能化策略被用于使UCNPs亲水、稳定和靶向细胞。用TEM、发射光谱、XRD、FTIR和zeta电位对制备的UCNPs进行了表征。用915 nm激光激发的特异性UCNPs显示出非常高对比度的UC生物成像。将高度稳定的dspe - mpeg -5000包封的UCNPs注射到小鼠体内进行成像。UC光致发光的成像和光谱分析表明，915 nm激光可以作为UC动物成像的一种新的有前景的激发光。
Successful further development of superhigh-constrast upconversion (UC) bioimaging requires addressing the existing paradox: 980 nm laser light is used to excite upconversion nanoparticles (UCNPs), while 980 nm light has strong optical absorption of water and biological specimens. The overheating caused by 980 nm excitation laser light in UC bioimaging is computationally and experimentally investigated for the first time. A new promising excitation approach for better near-infrared to near-infrared (NIR-to-NIR) UC photoluminescence in vitro or in vivo imaging is proposed employing a cost-effective 915 nm laser. This novel laser excitation method provides drastically less heating of the biological specimen and larger imaging depth In the animals or tissues due to quite low water absorption. Experimentally obtained thermal-graphic maps of the mouse in response to the laser heating are investigated to demonstrate the less heating advantage of the 915 nm laser. Our tissue phantom experiments and simulations verified that the 915 nm laser is superior to the 980 nm laser for deep tissue imaging. A novel and facile strategy for surface functionalization is utilized to render UCNPs hydrophilic, stable, and cell targeting. These as-prepared UCNPs were characterized by TEM, emission spectroscopy, XRD, FTIR, and zeta potential. Specifically targeting UCNPs excited with a 915 nm laser have shown very high contrast UC bioimaging. Highly stable DSPE-mPEG-5000-encapsulated UCNPs were injected into mice to perform in vivo imaging. Imaging and spectroscopy analysis of UC photoluminescence demonstrated that a 915 nm laser can serve as a new promising excitation light for UC animal imaging.