Colletotrichum higginsianum effectors exhibit cell to cell hypermobility in plant tissues and modulate intercellular connectivity amongst a variety of cellular processes
Colletotrichum higginsianum effectors exhibit cell to cell hypermobility in plant tissues and modulate intercellular connectivity amongst a variety of cellular processes
复制标题
DOI：
10.1101/2021.01.13.426415
复制
发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
M. Ohtsu;J. Jennings;M. Johnston;Xiaokun Liu;Nathan Hughes;K. Stark;R. Morris;Jeroen de Keijzer-Jeroen-de
中科院分区：
文献类型：
--
作者：
M. Ohtsu;J. Jennings;M. Johnston;Xiaokun Liu;Nathan Hughes;K. Stark;R. Morris;Jeroen de Keijzer-Jeroen-de
多细胞生物在细胞之间交换信息和资源以协调生长和反应。在植物中，胞间连丝在细胞之间建立细胞质连续性，以允许跨细胞壁的通讯和资源交换。一些植物病原体利用胞间连丝作为分子和物理入侵的途径。然而，人们对分子入侵（病原体效应子在细胞间移动）的好处知之甚少。为了开始研究这一点并确定哪些效应子是细胞间移动的，我们进行了基于实时成像的筛选，并确定了真菌病原体 Colletotrichum higginsianum 的 15 个细胞间移动效应子。其中，6 个是“超移动”的，显示出细胞间的移动性比其大小的蛋白质预期的要大。我们进一步确定了 3 个可以间接改变胞间连丝孔径的效应器。表达超活动效应子的植物的转录谱表明它们参与多种过程，包括衰老、硫代葡萄糖苷的产生、细胞壁完整性、生长和铁代谢。然而，并非所有效应器都对毒力产生独立影响。这表明希金斯梭菌效应器的移动性对感染有广泛的好处，这些效应器在感染过程中可能以复杂的方式相互作用。
Multicellular organisms exchange information and resources between cells to co-ordinate growth and responses. In plants, plasmodesmata establish cytoplasmic continuity between cells to allow for communication and resource exchange across the cell wall. Some plant pathogens use plasmodesmata as a pathway for both molecular and physical invasion. However, the benefits of molecular invasion (cell-to-cell movement of pathogen effectors) are poorly understood. To begin to investigate this and identify which effectors are cell-to-cell mobile, we performed a live imaging-based screen and identified 15 cell-to-cell mobile effectors of the fungal pathogen Colletotrichum higginsianum. Of these, 6 are “hypermobile”, showing cell-to-cell mobility greater than expected for a protein of its size. We further identified 3 effectors that can indirectly modify plasmodesmal aperture. Transcriptional profiling of plants expressing hypermobile effectors implicate them in a variety of processes including senescence, glucosinolate production, cell wall integrity, growth and iron metabolism. However, not all effectors had an independent effect on virulence. This suggests a wide range of benefits to infection gained by the mobility of C. higginsianum effectors that likely interact in a complex way during infection.