Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the MERTK locus
Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the MERTK locus
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DOI：
10.1016/j.scr.2021.102310
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发表时间：
2021
影响因子：
1.2
通讯作者：
Yizhi Liu
中科院分区：
文献类型：
--
作者：
Xiangcheng Tang;Jia-ling Fu;Zhigang Chen;Lixia Luo;David Wan-Cheng Li;Yizhi Liu
MERTK mutations are associate with rod-cone dystrophies. To enable investigations into the mechanism of this disease, we generated a cell line resource of H9 human embryonic stem cells harboring large fragment deletion mutation in a homozygous state in exon 19 of the MERTK gene. This subline expressed pluripotent stem cell markers, presented a normal karyotype, and preserved the ability to differentiate into endodermal, mesodermal, and ectodermal lineages.
