A UNIQUE SEQUENCE RELATED TO THE ECOTROPIC MURINE LEUKEMIA-VIRUS IS ASSOCIATED WITH THE FV-4 RESISTANCE GENE
A UNIQUE SEQUENCE RELATED TO THE ECOTROPIC MURINE LEUKEMIA-VIRUS IS ASSOCIATED WITH THE FV-4 RESISTANCE GENE
复制标题
DOI：
10.1073/pnas.81.3.834
复制
发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
BUCKLER, CE
中科院分区：
文献类型：
--
作者：
KOZAK, CA;GROMET, NJ;BUCKLER, CE
来自日本的几种实验室小鼠和野生小鼠品系在Fv-4位点（Fv-4 r）携带显性等位基因，该等位基因负责抵抗外源嗜亲性小鼠白血病病毒的感染。用亲嗜性病毒包膜特异性探针进行印迹杂交，结果表明，在日本小鼠Mus musculus molossinus和4个独立来源的携带Fv-4 r的部分同源株中存在一个独特的亲嗜性反应序列。对31只回交小鼠的分析表明，该片段的遗传与对Friend病毒复合体诱导的成红细胞增多症的抗性完全一致。该序列的遗传也抑制了M.臼齿肌限制性内切酶分析表明，Fv-4 r相关序列不同于实验室小鼠的全长亲嗜性前病毒。感染性病毒不能从仅携带Fv-4 r相关序列的小鼠中诱导。对其他野生小鼠抗Friend病毒复合物的检测表明，M。cervicolor cervicolor还含有Fv-4 r序列。显然，一个独特的或不完整的前病毒含有亲嗜性病毒相关序列是负责Fv-4介导的抵抗外源性和内源性亲嗜性病毒在各种亚洲小鼠。
Several strains of laboratory and wild-derived mice from Japan carry the dominant allele at the Fv-4 locus (Fv-4r) that is responsible for resistance to infection by exogenous ecotropic murine leukemia virus. Blot hybridization with a probe specific for the ecotropic viral envelope was used to show that a unique envelope-reactive sequence is present in the Japanese mouse Mus musculus molossinus and in 4 independently derived partially congeneic strains carrying Fv-4r. Analysis of 31 backcross mice shows complete concordance between inheritance of this fragment and resistance to Friend virus complex-induced erythroblastosis. Inheritance of this sequence also suppresses spontaneous expression of the endogenous ecotropic viruses carried by M. musculus molossinus. Restriction enzyme analysis shows that that Fv-4r-associated sequence is different from the full-length ecotropic proviruses of laboratory mice. Infectious virus cannot be induced from mice carrying only the Fv-4r-associated sequence. Examination of other wild-derived mice resistant to Friend virus complex shows that M. cervicolor cervicolor also contains the Fv-4r sequence. Evidently, a unique or incomplete provirus containing ecotropic envelope-related sequences is responsible for Fv-4-mediated resistance to both exogenous and endogenous ecotropic virus in various Asian mice.