Murine cytotoxic T lymphocytes specific for herpes simplex virus type 1 recognize the immediate early protein ICP4 but not ICP0.
Murine cytotoxic T lymphocytes specific for herpes simplex virus type 1 recognize the immediate early protein ICP4 but not ICP0.
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DOI：
10.1099/0022-1317-71-10-2391
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发表时间：
1990-10
期刊：
影响因子：
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通讯作者：
S. Martin;X. Zhu;S. Silverstein;R. Courtney;F. Yao;F. Jenkins;B. Rouse
中科院分区：
文献类型：
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作者：
S. Martin;X. Zhu;S. Silverstein;R. Courtney;F. Yao;F. Jenkins;B. Rouse
使用表达编码ICP 0或ICP 4的单纯疱疹病毒1型（HSV-1）基因的牛痘病毒重组体来鉴定对非结构立即早期蛋白具有特异性的鼠抗病毒细胞毒性T淋巴细胞（CTL）的精确靶抗原。这些研究表明，仅限于H-2k单倍型而不是H-2d或H-2b单倍型的I类主要组织相容性复合体基因的HSV-1特异性CTL会溶解表达ICP 4的自体细胞。来自不同小鼠品系的HSV-1特异性CTL不能裂解表达ICP 0的靶细胞。H-2k限制性病毒特异性CTL频率的计算表明，约三分之一的总HSV-1特异性应答针对ICP 4。用重组牛痘病毒或表达ICP 4的转染L细胞免疫小鼠可诱导HSV-1特异性淋巴细胞增殖和迟发型超敏反应，但未诱导CTL。更重要的是，这种免疫的动物不能抵抗或控制随后用毒性HSV-1的攻击。
Vaccinia virus recombinants expressing the herpes simplex virus type 1 (HSV-1) genes encoding ICP0 or ICP4 were used to identify the precise target antigen(s) of murine anti-viral cytotoxic T lymphocytes (CTL) specific for the non-structural immediate early proteins. These studies revealed that HSV-1-specific CTL, restricted to class I major histocompatibility complex genes of the H-2k haplotype but not the H-2d or H-2b haplotypes, would lyse autologous cells expressing ICP4. HSV-1-specific CTL derived from various mice strains failed to lyse target cells expressing ICP0. Calculation of the frequencies of H-2k-restricted virus-specific CTL demonstrated that approximately a third of the total HSV-1-specific response was directed against ICP4. Immunization of mice with either recombinant vaccinia virus or transfected L cells expressing ICP4 induced HSV-1-specific lymphoproliferation and delayed hypersensitivity but CTLs were not induced. More importantly, such immunized animals were unable to resist or control a subsequent challenge with virulent HSV-1.