Isolation of foot-and-mouth disease virus from Japanese Black cattle in Miyazaki Prefecture, Japan, 2000
Isolation of foot-and-mouth disease virus from Japanese Black cattle in Miyazaki Prefecture, Japan, 2000
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DOI：
10.1292/jvms.64.91
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发表时间：
2002-01-01
影响因子：
1.2
通讯作者：
Murakami, Y
中科院分区：
文献类型：
--
作者：
Sakamoto, K;Kanno, T;Murakami, Y
2000年3月至5月，日本宫崎县和北海道县发生了4起口蹄疫疫情。从宫崎县发现的第3例日本黑牛中采集病料，接种于牛肾（BK）和牛甲状腺细胞培养物中，分离出口蹄疫病毒。接种后2天，在BK中观察到CPE。用逆转录聚合酶链反应（RT-PCR）检测培养液中口蹄疫病毒（FMDV）特异性扩增片段。用酶联免疫吸附试验（ELISA）检测抗原，鉴定为0型口蹄疫病毒，并测定了编码VP 1的核苷酸序列。该口蹄疫病毒是一种在泛亚洲广泛传播的毒株，被英国皮尔布赖特研究所世界参考实验室指定为O/JPN/2000。这份报告标志着日本首次分离出FMDV。
Four outbreaks of foot-and-mouth disease (FMD) occurred from March to May 2000 in Miyazaki and Hokkaido Prefectures, Japan. FMD virus isolation was achieved by sampling probang materials from Japanese Black cattle in the third case found in Miyazaki Prefecture, The probang materials were inoculated to bovine kidney (BK) and bovine thyroid cell cultures. CPE was observed in the BK at two days post-inoculation. Specific amplified DNA segments for FMD virus (FMDV) were detected by reverse transcriptase polymerase chain reaction in the culture fluid. The FMDV was identified as type 0 by enzyme-linked immunosorbent assay (ELISA) for antigen detection and the nucleotide sequence encoding the VP1 was determined. This FMDV is a strain that is widespread in Pan-Asia and was designated as O/JPN/2000 by the World Reference Laboratory of the Pirbright Institute, England. This report marks the first isolation of FMDV in Japan.