In situ control of cell adhesion using photoresponsive culture surface
In situ control of cell adhesion using photoresponsive culture surface
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DOI：
10.1021/bm0493382
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发表时间：
2005-03-01
影响因子：
6.2
通讯作者：
Yoshimi, Y
中科院分区：
文献类型：
--
作者：
Edahiro, J;Sumaru, K;Yoshimi, Y
以含有螺吡喃发色团的聚N-异丙基丙烯酰胺为侧链的新型聚合物材料制备了一种可光控制细胞黏附的光响应培养表面(PRCS)。用紫外光(波长：365 nm)照射后，细胞在表面的粘附性显著增强；随后的冷却和冰洗后，许多细胞仍然留在照射区域，而大部分细胞从非照射区域被移除。用可见光(波长400~440 nm)照射，37℃保温2 h后，细胞黏附被紫外光照射增强，细胞黏附的区域调控被多次重复。此外，通过将紫外光沿着预先均匀播种活细胞的PRCS上的微图案投射，可以容易地产生具有200微米线宽的活细胞图案。通过使用仅对活细胞进行染色的荧光探针，证实即使在紫外线照射后冷却和清洗后，细胞仍保持足够的活力。
A photoresponsive culture surface (PRCS) allowing photocontrol of cell adhesion was prepared with a novel polymer material composed of poly(N-isopropylacrylamide) having spiropyran chromophores as side chains. Cell adhesion of the surface was drastically enhanced by the irradiation with ultraviolet (UV) light (wavelength: 365 nm); after subsequent cooling and washing on ice, many cells remained in the irradiated region, whereas most cells were removed from the nonirradiated region. The cell adhesion of the PRCS, which had been enhanced by previous UV irradiation, was reset by the visible light irradiation (wavelength 400-440 nm) and the annealing at 37 degrees C for 2 h. Also it was confirmed that the regional control of cell adhesion was induced several times by repeating the same series of operations. Further, living cell patterning with the 200 mu m line width was produced readily by projecting UV light along a micropattern on the PRCS on which the living cells had been seeded uniformly in advance. By using a fluorescent probe that stains living cells only, it was confirmed that the cells maintained sufficient viability even after UV light irradiation followed by cooling and washing.