Novel Photodynamic Therapy Using Water-dispersed TiO2-Polyethylene Glycol Compound: Evaluation of Antitumor Effect on Glioma Cells and Spheroids In Vitro
Novel Photodynamic Therapy Using Water-dispersed TiO2-Polyethylene Glycol Compound: Evaluation of Antitumor Effect on Glioma Cells and Spheroids In Vitro
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DOI：
10.1111/j.1751-1097.2010.00742.x
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发表时间：
2010-07-01
影响因子：
3.3
通讯作者：
Iwasaki, Yoshinobu
中科院分区：
文献类型：
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作者：
Yamaguchi, Shigeru;Kobayashi, Hiroyuki;Iwasaki, Yoshinobu
二氧化钛(Ti02)被认为是一种受紫外光激发的光催化剂。我们的目的是研究水分散的二氧化钛纳米粒子对C6大鼠胶质瘤细胞的光催化抗肿瘤作用，并通过球形模型评价其治疗效果。将化学修饰的聚乙二醇单分散在二氧化钛表面，制备了水分散的二氧化钛纳米粒子。C6细胞的每个单层和球体与不同浓度的二氧化钛/聚乙二醇共孵育，然后用紫外光照射。用荧光染料连续染色观察细胞和球体的损伤情况。细胞毒作用与二氧化钛/聚乙二醇的浓度和紫外光照射的能量剂量有关。在500微克毫克/毫升的二氧化钛/聚乙二醇存在下，紫外光照射13.5J·cm~(-2)后，细胞的杀伤率达90%以上。与对照组相比，照射后的球体在Ti02/PEG存在下表现出生长抑制。在处理后的球体中，Annexin V-FITC染色的细胞数在前6h逐渐增多，随后出现碘化丙啶染色的细胞。本研究结果表明，新开发的光激发二氧化钛/聚乙二醇具有一定的抗肿瘤活性。利用这种材料的光动力疗法可以为胶质瘤的新治疗策略提供线索。
Titanium dioxide (TiO2) is thought to be a photocatalytic agent excited by UV light. Our aim was to investigate the photocatalytic antitumor effect of water-dispersed TiO2 nanoparticles on C6 rat glioma cells and to evaluate the treatment responses by the spheroid models. Water-dispersed TiO2 nanoparticles were constructed by the adsorption of chemical modified polyethylene glycol (PEG) on the TiO2 surface (TiO2/PEG). Each monolayer and spheroid of C6 cells was coincubated with various concentrations of TiO2/PEG and subsequently irradiated with UV light. Damage of the cells and spheroids was evaluated sequentially by staining with the fluorescent dyes. The cytotoxic effect was correlated with the concentration of TiO2/PEG and the energy dose of UV irradiation. More than 90% of cells were killed after 13.5 J cm-2 of UV irradiation in the presence of 500 mu g mL-1 TiO2/PEG. The irradiated spheroids in the presence of TiO2/PEG showed growth suppression compared with control groups. In TiO2/PEG-treated spheroids, the number of Annexin V-FITC-stained cells gradually increased during the first 6 h, and subsequently propidium iodide-stained cells appeared. The results of this study suggest that newly developed photoexcited TiO2/PEG have antitumoral activity. Photodynamic therapy utilizing this material can be a clue to a novel therapeutic strategy for glioma.