Localization of ginsenosides in Panax ginseng with different age by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry imaging
Localization of ginsenosides in Panax ginseng with different age by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry imaging
复制标题
基质辅助激光解吸/电离飞行时间质谱成像对不同树龄人参中人参皂苷的定位
DOI：
10.1016/j.jchromb.2015.09.024
复制
发表时间：
2016-07-15
影响因子：
3
通讯作者：
Cai, Zongwei
中科院分区：
文献类型：
--
作者：
Bai, Hangrui;Wang, Shujuan;Cai, Zongwei
人参的根。人参（P. ginseng）是最受欢迎的中药之一，人参皂苷是其主要的生物活性成分。由于不同人参皂苷具有不同的药理作用，因此研究不同人参皂苷的药理作用通常需要对人参皂苷进行提取和分离。人参皂苷的含量随人参根龄和组织的不同而不同。本研究建立了一种基于基质辅助激光解吸/电离飞行时间质谱成像（MALDI-TOF-MSI）的有效方法来探索人参皂苷的分布并区分不同年龄的人参根。经过简单的样品制备，通过MALDI-TOF-MSI和MALDI-TOF-MS/MS鉴定，在m/z 700 ~ 1400的质量范围内，有18个峰对应31种人参皂苷，具有明确的定位。三种人参皂苷均被成功地检测到，并在图像中显示出来，与解剖特征具有相关性。对2、4、6龄人参的离子图像数据进行主成分分析，可以很好地区分出人参的不同年龄，但对整个组织的数据却不能很好地区分。实验结果表明，建立的植物组织代谢物直接分析方法在药材代谢物的快速鉴定和定位分析方面具有很大的潜力。此外，该技术还为草药的成分特异性提取和药理研究提供了有价值的信息。(C) 2015 Elsevier B.V.版权所有
The root of Panax ginseng C.A. Mey. (P. ginseng) is one of the most popular traditional Chinese medicines, with ginsenosides as its main bioactive components. Because different ginsenosides have varied pharmacological effects, extraction and separation of ginsenosides are usually required for the investigation of pharmacological effects of different ginsenosides. However, the contents of ginsenosides vary with the ages and tissues of P. ginseng root. In this research, an efficient method to explore the distribution of ginsenosides and differentiate P. ginseng roots with different ages was developed based on matrix assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF-MSI). After a simple sample preparation, there were 18 peaks corresponding to 31 ginsenosides with distinct localization in the mass range of m/z 700-1400 identified by MALDI-TOF-MSI and MALDI-TOF-MS/MS. All the three types of ginsenosides were successfully detected and visualized in images, which could be correlated with anatomical features. The P. ginseng at the ages of 2, 4 and 6 could be differentiated finely through the principal component analysis of data collected from the cork based on the ion images but not data from the whole tissue. The experimental result implies that the established method for the direct analysis of metabolites in plant tissues has high potential for the rapid identification of metabolites and analysis of their localizations in medicinal herbs. Furthermore, this technique also provides valuable information for the component-specific extraction and pharmacological research of herbs. (C) 2015 Elsevier B.V. All rights reserved.