Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation.
Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation.
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DOI：
10.1371/journal.pone.0020238
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Jiao B
中科院分区：
文献类型：
--
作者：
Chen H;Sun Y;Dong R;Yang S;Pan C;Xiang D;Miao M;Jiao B
MicroRNA是一类小的调节RNA，其调节多种生物过程，包括细胞分化、凋亡、代谢和增殖。本研究旨在探讨miR-34 a对肝细胞增殖的影响及其在肝再生终止中的潜在作用。miR-34 a在部分肝切除术后高度诱导。miR-34 a在BRL-3A细胞中的过表达可显著抑制细胞增殖，并下调BRL-3A细胞中IFN-βB（INHB B）和Met的表达。在BRL-3A细胞中，通过荧光素酶报告基因测定，INHBB被鉴定为miR-34 a的直接靶点。更重要的是，INHBB siRNA显著抑制细胞增殖。在再生肝中检测到INHBB和Met的减少。miR-34 a的表达在肝再生的晚期阶段上调。miR-34 a介导的INHBB和Met调节可能共同有助于抑制肝细胞增殖。
MicroRNAs are a class of small regulatory RNAs that modulate a variety of biological processes, including cellular differentiation, apoptosis, metabolism and proliferation. This study aims to explore the effect of miR-34a in hepatocyte proliferation and its potential role in liver regeneration termination. MiR-34a was highly induced after partial hepatectomy. Overexpression of miR-34a in BRL-3A cells could significantly inhibit cell proliferation and down-regulate the expression of inhibin βB (INHBB) and Met. In BRL-3A cells, INHBB was identified as a direct target of miR-34a by luciferase reporter assay. More importantly, INHBB siRNA significantly repressed cell proliferation. A decrease of INHBB and Met was detected in regenerating liver. MiR-34a expression was upregulated during the late phase of liver regeneration. MiR-34a-mediated regulation of INHBB and Met may collectively contribute to the suppression of hepatocyte proliferation.
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影响因子：
3.7
作者：
Factor, Valentina M.;Seo, Daekwan;Thorgeirsson, Snorri S.
通讯作者：
Thorgeirsson, Snorri S.
影响因子：
13.5
作者：
Apte, Udayan;Gkretsi, Vasiliki;Bowen, William C.;Mars, Wendy M.;Luo, Jian-Hua;Donthamsetty, Shashikiran;Orr, Ann;Monga, Satdarshan P. S.;Wu, Chuanyue;Michalopoulos, George K.
通讯作者：
Michalopoulos, George K.
影响因子：
13.5
作者：
Oe, S;Lemmer, ER;Thorgeirsson, SS
通讯作者：
Thorgeirsson, SS
影响因子：
13.5
作者：
Fausto, N;Campbell, JS;Riehle, KJ
通讯作者：
Riehle, KJ
DOI：
10.1111/igc.0b013e3181d63170
发表时间：
2010-05-01
影响因子：
4.8
作者：
Li, Baohua;Hu, Ying;Xie, Xing
通讯作者：
Xie, Xing