Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT
Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT
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DOI：
10.1111/2049-632x.12041
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发表时间：
2013-06-01
影响因子：
3.3
通讯作者：
Takashiba, Shogo
中科院分区：
文献类型：
--
作者：
Hirai, Kimito;Maeda, Hiroshi;Takashiba, Shogo
组I（HSP 60）和组II（CCT）伴侣蛋白都是自身抗体的靶标。对HSP 60的自身免疫反应已得到很好的表征，而对II组伴侣蛋白的免疫反应尚未阐明。口腔甲烷短杆菌是一种可疑的牙周致病菌与组II伴侣。在这项研究中，使用牙周炎和自身免疫性疾病患者的血清检测了对口腔支原体伴侣蛋白、人HSP 60和CCT亚基的血清反应。与健康对照组相比，牙周炎患者对CCT 4和CCT 8的斑点印迹分析显示出显着更高的反应。自身免疫性疾病患者中CCT 3和CCT 8的信号显著高于对照组。免疫印迹法也证实了两个患者组中抗CCT 4应答的显著差异。所有受试者对口腔支原体伴侣蛋白表现出强烈的反应性，对人HSP 60表现出微弱的信号。针对CCT而不是HSP 60产生自身抗体;并且显示CCT 3、CCT 4和CCT 8是主要靶标。宿主免疫系统可能经常暴露于各种栖息地的寄生虫伴侣蛋白。虽然需要进一步研究口腔支原体伴侣蛋白和人CCT之间的交叉反应性，但抗CCT自身抗体可能参与牙周炎和自身免疫性疾病的发病机制。
Both group I (HSP60) and group II (CCT) chaperonins are targets of autoantibodies. Autoimmune reactions to HSP60 have been well characterized, while immune reactions to group II chaperonin have not been clarified. Methanobrevibacter oralis is a suspected periodontal pathogen with group II chaperonin. In this study, serum responses to M.oralis chaperonin, human HSP60, and CCT subunits were examined using sera from patients with periodontitis and autoimmune diseases. In comparison with healthy controls, periodontitis patients showed significantly higher responses to CCT4 and CCT8 on dot blot analysis. Signals for CCT3 and CCT8 in autoimmune disease patients were significantly higher than in controls. Significant differences were also demonstrated by Western blotting in anti-CCT4 response in both patient groups. All subjects showed strong reactivity to M.oralis chaperonin and faint signals to human HSP60. Autoantibodies were raised against CCT rather than HSP60; and CCT3, CCT4, and CCT8 were shown to be the main targets. Host immune systems may be frequently exposed to chaperonins of Archaea in various habitats. Although further studies of the cross-reactivity between M.oralis chaperonin and human CCT are required, anti-CCT autoantibodies may be involved in the pathogenesis of periodontitis and autoimmune diseases.