Dendrites of medial olivocochlear neurons in mouse
Dendrites of medial olivocochlear neurons in mouse
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DOI：
10.1016/j.neuroscience.2007.12.045
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发表时间：
2008-06-12
期刊：
影响因子：
3.3
通讯作者：
Levine, J. L.
中科院分区：
文献类型：
--
作者：
Brown, M. C.;Levine, J. L.
用乙酰胆碱酯酶（ACNE）染色和荧光金（FG）逆行标记法研究了小鼠橄榄耳蜗神经元及其树突的形态。这两种方法在胞体的位置和数量上得到了相似的结果。在斜方体腹侧核（VNTB）中，内侧橄榄耳蜗（MOC）神经元的总数约为每侧170个。另外十几个大的橄榄耳蜗神经元位于背侧橄榄周围核（DPO）。所有这些神经元的树突都很长，从细胞体向各个方向延伸，这种模式与它们反应的尖锐频率调谐形成对比。对于VNTB神经元，有更多的树突指向内侧比外侧和那些指向内侧更长（平均，25-50%长）。位于VNTB的吻侧部分的神经元的树突延伸是最明显的。当来自单个神经元的每个树突被表示为一个向量，并且所有向量相加时，结果也向内侧方向倾斜。然而，DPO神经元有更多的对称树突投射到梯形体的更多背侧部分，这表明这一小群橄榄耳蜗神经元具有非常不同的生理特性。这两种类型的神经元的树突有点伸长吻，约20%的长度比那些指向尾部。这些结果可以被解释为橄榄耳蜗神经元树突向其突触输入的延伸：内侧满足来自耳蜗核的交叉纤维，这是MOC反射通路的一部分，和嘴侧满足来自更高中心的下行输入。(C)2008年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Stains for acetylcholinesterase (ACNE) and retrograde labeling with Fluorogold (FG) were used to study olivocochlear neurons and their dendritic patterns in mice. The two methods gave similar results for location and number of somata. The total number of medial olivocochlear (MOC) neurons in the ventral nucleus of the trapezoid body (VNTB) is about 170 per side. An additional dozen large olivocochlear neurons are located in the dorsal periolivary nucleus (DPO). Dendrites of all of these neurons are long and extend in all directions from the cell bodies, a pattern that contrasts with the sharp frequency tuning of their responses. For VNTB neurons, there were greater numbers of dendrites directed medially than laterally and those directed medially were longer (on average, 25-50% longer). Dendrite extensions were most pronounced for neurons located in the rostral portion of the VNTB. When each dendrite from a single neuron was represented as a vector, and all the vectors summed, the result was also skewed toward the medial direction. DPO neurons, however, had more symmetric dendrites that projected into more dorsal parts of the trapezoid body, suggesting that this small group of olivocochlear neurons has very different physiological properties. Dendrites of both types of neurons were somewhat elongated rostrally, about 20% longer than those directed caudally. These results can be interpreted as extensions of dendrites of olivocochlear neurons toward their synaptic inputs: medially to meet crossing fibers from the cochlear nucleus that are part of the MOC reflex pathway, and rostrally to meet descending inputs from higher centers. (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.