Digital light processing 3D printed silk fibroin hydrogel for cartilage tissue engineering
Digital light processing 3D printed silk fibroin hydrogel for cartilage tissue engineering
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DOI：
10.1016/j.biomaterials.2019.119679
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发表时间：
2020-02-01
期刊：
影响因子：
14
通讯作者：
Park, Chan Hum
中科院分区：
文献类型：
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作者：
Hong, Heesun;Seo, Ye Been;Park, Chan Hum
利用数字照明处理(DLP)打印机进行三维打印已成为再生医学组织工程领域的新浪潮。尤其是在临床应用方面，需要开发具有生物相容性、生物可降解性和可印刷性的生物墨水。因此，我们证明了丝素蛋白作为一种天然聚合物由甲基丙烯酸缩水甘油酯(Silk-GMA)制备而成，用于DLP 3D打印。体外培养系统和体内应用对负载软骨细胞的Silk-GMA的软骨形成能力进行评价。由于DLP 3D打印机的快速打印速度和光聚合，DLP 3D打印系统为3D产品提供了均匀的细胞分布。Silk-GMA水凝胶体外培养长达4周，可确保被包裹细胞的存活、增殖和向软骨分化。此外，对部分气管损伤的兔模型进行的体内实验表明，移植的Silk-GMA水凝胶周围有新的软骨样组织和上皮细胞。利用DLP 3D打印机制作的Silk GMA水凝胶有望应用于软骨再生等需要力学性能的组织工程领域。
Three-dimensional printing with Digital Lighting Processing (DLP) printer has come into the new wave in the tissue engineering for regenerative medicine. Especially for the clinical application, it needs to develop of bio-ink with biocompatibility, biodegradability and printability. Therefore, we demonstrated that Silk fibroin as a natural polymer fabricated with glycidyl-methacrylate (Silk-GMA) for DLP 3D printing. The ability of chondrogenesis with chondrocyte-laden Silk-GMA evaluated in vitro culture system and applied in vivo. DLP 3D printing system provided 3D product with even cell distribution due to rapid printing speed and photopolymerization of DLP 3D printer. Up to 4 weeks in vitro cultivation of Silk-GMA hydrogel allows to ensure of viability, proliferation and differentiation to chondrogenesis of encapsulated cells. Moreover, in vivo experiments against partially defected trachea rabbit model demonstrated that new cartilage like tissue and epithelium found surrounding transplanted Silk-GMA hydrogel. This study promises the fabricated Silk GMA hydrogel using DLP 3D printer could be applied to the fields of tissue engineering needing mechanical properties like cartilage regeneration.