Pigment-dispersing factor is involved in photoperiodic control of reproduction in the brown-winged green bug, Plautia stali
Pigment-dispersing factor is involved in photoperiodic control of reproduction in the brown-winged green bug, Plautia stali
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DOI：
10.1016/j.jinsphys.2022.104359
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发表时间：
2022-01-22
影响因子：
2.2
通讯作者：
Shiga, Sakiko
中科院分区：
文献类型：
--
作者：
Hasebe, Masaharu;Kotaki, Toyomi;Shiga, Sakiko
温带地区的动物通过光周期性感知季节变化，在适当的季节繁殖。许多研究表明，昼夜节律钟系统参与光周期调节生殖。色素分散因子（PDF）是一种已知的脑神经肽，参与各种昆虫的昼夜节律控制。在这里，我们调查的本地化和投影的PDF神经元在大脑中，他们参与的光周期控制繁殖的棕色翅膀的绿色错误，Plautia stali的女性。免疫组织化学分析显示，密集的聚偏二氟乙烯免疫反应细胞集中在近端髓质的视叶，这对应于集群被称为PDFMe细胞。PDGF免疫反应阳性细胞通过髓质表面向板层投射纤维。PDF免疫反应纤维也被发现在前脑，似乎连接两个PDF细胞体的视叶。RNA干扰介导的敲低pdf抑制了由长日照条件转移到短日照条件引起的产卵停滞。此外，敲低pdf延迟产卵开始后，从短到长的一天条件的变化。总之，研究结果表明PDF在近端骨髓的细胞簇中局部表达，并参与P. stali雌性生殖的光周期控制。
Animals in temperate regions breed in the appropriate season by sensing seasonal changes through photoperiodism. Many studies suggest the involvement of a circadian clock system in the photoperiodic regulation of reproduction. Pigment-dispersing factor (PDF) is a known brain neuropeptide involved in the circadian control in various insects. Here, we investigated the localization and projection of PDF neurons in the brain and their involvement in the photoperiodic control of reproduction in the females of the brown-winged green bug, Plautia stali. Immunohistochemical analyses revealed a dense cluster of PDF-immunoreactive cells localized in the proximal medulla of the optic lobe, which corresponded to the cluster known as PDFMe cells. PDFimmunoreactive cells projected their fibers to the lamina through the medulla surface. PDF-immunoreactive fibers were also found in the protocerebrum and seemed to connect both PDF cell bodies in the optic lobes. RNA interference-mediated knockdown of pdf inhibited oviposition arrest induced by the transfer from long-to short-day conditions. Additionally, the knockdown of pdf delayed oviposition onset after the change from short to long-day conditions. In conclusion, the study results indicate that PDF is locally expressed in a cell cluster at the proximal medulla and involved in the photoperiodic control of reproduction in P. stali females.