Micro-drive array for chronic in vivo recording: drive fabrication.
Micro-drive array for chronic in vivo recording: drive fabrication.
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DOI：
10.3791/1094
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发表时间：
2009-04-20
期刊：
影响因子：
--
通讯作者：
Wilson, Matthew A
中科院分区：
文献类型：
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作者：
Kloosterman, Fabian;Davidson, Thomas J;Wilson, Matthew A
慢性记录大量神经元是研究清醒行为大鼠神经元回路功能的一种有价值的技术。携带高密度四极阵列的轻型记录设备允许同时监测数十至数百个单个神经元的活动。在这里，我们描述了一个协议，用于制造的微驱动器阵列与21个独立可移动的微驱动器。该装置已成功地用于本实验室海马和皮层神经元的记录。我们将展示如何准备一个定制设计的，3D打印的塑料底座，以容纳微型驱动器。我们演示了如何构建单个微驱动器以及如何组装完整的微驱动器阵列。在后续的视频文章中将介绍用于外科植入的驱动阵列的进一步准备，例如四极的制造、四极到驱动阵列中的装载和镀金。
Chronic recording of large populations of neurons is a valuable technique for studying the function of neuronal circuits in awake behaving rats. Lightweight recording devices carrying a high density array of tetrodes allow for the simultaneous monitoring of the activity of tens to hundreds of individual neurons. Here we describe a protocol for the fabrication of a micro-drive array with twenty one independently movable micro-drives. This device has been used successfully to record from hippocampal and cortical neurons in our lab. We show how to prepare a custom designed, 3-D printed plastic base that will hold the micro-drives. We demonstrate how to construct the individual micro-drives and how to assemble the complete micro-drive array. Further preparation of the drive array for surgical implantation, such as the fabrication of tetrodes, loading of tetrodes into the drive array and gold-plating, is covered in a subsequent video article.