Potential TMA-Producing Bacteria Are Ubiquitously Found in Mammalia
Potential TMA-Producing Bacteria Are Ubiquitously Found in Mammalia
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DOI：
10.3389/fmicb.2019.02966
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发表时间：
2020-01-09
影响因子：
5.2
通讯作者：
Vital, Marius
中科院分区：
文献类型：
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作者：
Rath, Silke;Rud, Tatjana;Vital, Marius
人体肠道细菌将胆碱和肉碱等饮食成分代谢成三甲胺(TMA)，三甲胺随后被肝酶氧化成三甲胺-N-氧化物(TMAO)。血浆TMAO水平升高与心血管和肾脏疾病的发生有关。在这项研究中，我们应用基因靶向分析来定量(QPCR)和表征(MiSeq)编码TMA产生的酶，即胆碱-TMA裂解酶(CutC)、肉碱加氧酶(CNTA)和甜菜碱还原酶(GrdH)的细菌基因，这些基因来自不同哺乳动物的粪便样本，这些样本来自三个饮食群体(肉食性动物、杂食性动物和草食性动物)和四个寄主目(食肉目、灵长目、偶蹄目和围足目)。然而，所有样品中都含有潜在的TMA产生菌，但丰度较低(
Human gut bacteria metabolize dietary components such as choline and carnitine to trimethylamine (TMA) that is subsequently oxidized to trimethylamine-N-oxide (TMAO) by hepatic enzymes. Increased plasma levels of TMAO are associated with the development of cardiovascular and renal disease. In this study, we applied gene-targeted assays in order to quantify (qPCR) and characterize (MiSeq) bacterial genes encoding enzymes responsible for TMA production, namely choline-TMA lyase (CutC), carnitine oxygenase (CntA) and betaine reductase (GrdH) in 89 fecal samples derived from various mammals spanning three dietary groups (carnivores, omnivores and herbivores) and four host orders (Carnivora, Primates, Artiodactyla and Perissodactyla). All samples contained potential TMA-producing bacteria, however, at low abundances (