Modelling real-time simultaneous saccharification and fermentation of lignocellulosic biomass and organic acid accumulation using dielectric spectroscopy
Modelling real-time simultaneous saccharification and fermentation of lignocellulosic biomass and organic acid accumulation using dielectric spectroscopy
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DOI：
10.1016/j.biortech.2011.07.084
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发表时间：
2011-10-01
影响因子：
11.4
通讯作者：
Gallagher, Joe A.
中科院分区：
文献类型：
--
作者：
Bryant, David N.;Morris, Stephen M.;Gallagher, Joe A.
介电谱 (DS) 通常用于酵母和哺乳动物发酵，通过活细胞的固有电容定量监测活生物量：然而，使用 DS 监测木质纤维素生物量的酶分解尚未见报道。本研究的目的是检验 DS 在监测高糖多年生黑麦草 (HS-PRG) 纤维酶促糖化中的应用，并将数据与化学成分的变化联系起来。 DS 能够在线监测酶水解过程中 PRG 纤维电容 (C = 580 kHz) 的下降，以及随后微生物生长过程中有机酸产生导致的电导率增加 (G = 580 kHz)。纤维组分分析显示，>50% 的 HS-PRG 木质纤维素已经历酶水解。这些数据证明了 DS 生物量探针在同步糖化发酵 (SSF) 在线监测中的实用性。 (C) 2011 Elsevier Ltd. 保留所有权利。
Dielectric spectroscopy (DS) is routinely used in yeast and mammalian fermentations to quantitatively monitor viable biomass through the inherent capacitance of live cells: however, the use of DS to monitor the enzymatic break down of lignocellulosic biomass has not been reported. The aim of the current study was to examine the application of DS in monitoring the enzymatic saccharification of high sugar perennial ryegrass (HS-PRG) fibre and to relate the data to changes in chemical composition. DS was capable of both monitoring the on-line decrease in PRG fibre capacitance (C = 580 kHz) during enzymatic hydrolysis, together with the subsequent increase in conductivity (G = 580 kHz) resulting from the production of organic acids during microbial growth. Analysis of the fibre fractions revealed >50% of HS-PRG lignocellulose had undergone enzymatic hydrolysis. These data demonstrated the utility of DS biomass probes for on-line monitoring of simultaneous saccharification and fermentation (SSF). (C) 2011 Elsevier Ltd. All rights reserved.