Asymmetric expression of maternal mRNA governs first cell-fate decision
Asymmetric expression of maternal mRNA governs first cell-fate decision
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母体 mRNA 的不对称表达控制着第一个细胞命运的决定
DOI：
10.1096/fj.202101196r
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发表时间：
2021
期刊：
影响因子：
4.8
通讯作者：
Xu Liu
中科院分区：
文献类型：
--
作者：
Yingmei Wang;Xu Liu
植入前发育的目标是确定胚胎和胚外细胞的命运。然而，在早期哺乳动物胚胎发育过程中，细胞命运何时以及如何决定仍不清楚。我们报告了高迁移率族（HMG）蛋白家族成员HMGA1在小鼠两细胞卵裂球中的分布差异。两个细胞之一中HMGA 1表达的敲减减少了构成内细胞团（ICM）的细胞数量，这表明HMGA 1在双细胞小鼠胚胎的卵裂球中的差异分布影响了胚胎细胞谱系的选择。从机制上讲，HMGA1促进ICM特异性基因Sox 2的表达。这项研究的结果表明，小鼠胚胎早在两细胞阶段就表现出异质性，这些差异与早期小鼠胚胎的细胞命运分化有关。
The goal of preimplantation development is to establish the fates of the embryonic and extra‐embryonic cells. However, when and how cell fates are determined during early mammalian embryonic development remains unclear. We report that the high mobility group (HMG) protein family member HMGA1 was distributed differentially in mouse two‐cell blastomeres. Knockdown ofHmga1expression in one of the two cells reduced the number of cells contributing to the inner cell mass (ICM), suggesting that differential distribution of HMGA1 in the blastomeres in two‐cell mouse embryos affected the selection of embryonic cell lineages. Mechanistically, HMGA1 promotes the expression of the ICM‐specific geneSox2. The results of this study show that mouse embryos demonstrate heterogeneity as early as the two‐cell stage, and that these differences are related to cell‐fate differentiation in early mouse embryos.