BRIEF, HIGH-TEMPERATURE HEAT DENATURATION (PRESSURE COOKING) - A SIMPLE AND EFFECTIVE METHOD OF ANTIGEN RETRIEVAL FOR ROUTINELY PROCESSED TISSUES
BRIEF, HIGH-TEMPERATURE HEAT DENATURATION (PRESSURE COOKING) - A SIMPLE AND EFFECTIVE METHOD OF ANTIGEN RETRIEVAL FOR ROUTINELY PROCESSED TISSUES
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DOI：
10.1002/path.1711730413
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发表时间：
1994-08-01
影响因子：
7.3
通讯作者：
YEOMANS, P
中科院分区：
文献类型：
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作者：
NORTON, AJ;JORDAN, S;YEOMANS, P
最近强调了基于微波的抗原修复方法用于诊断性免疫组织化学的优点。由于此类技术对于繁忙的常规实验室存在缺点，因此我们研究了另一种热介导抗原修复 (HMAR) 方法，即使用家用高压锅作为过热源。一个简单的方案，在 0.01 M 柠檬酸盐缓冲液（pH 6.0）中过热 2 分钟，得到的结果与微波炉中获得的结果相当。回收的抗原范围和加热无效的抗原与已发表的微波经验相匹配。其优点包括处理速度、大批量载玻片结果的可重复性、使用金属载玻片架的能力以及时间和设备成本的经济性。
The merits of microwave-based antigen retrieval methods for diagnostic immunohistochemistry have recently been emphasized. As such technology has drawbacks for busy routine laboratories, we have investigated an alternative method of heat-mediated antigen retrieval (HMAR), using a domestic pressure cooker as a source of superheating. A simple protocol, with superheating in 0.01 M citrate buffer (pH 6.0) for 2 min, gave results comparable to those obtainable in a microwave oven. The range of antigens retrieved and the antigens for which heating was ineffective matched the published microwave experience. The advantages included the speed of treatment, the reproducibility of results with large batches of slides, the ability to use metal slide racks, and economy of time and equipment costs.