Intracellular free magnesium and high energy phosphates in the perfused normotensive and spontaneously hypertensive rat heart. A 31P NMR study.
Intracellular free magnesium and high energy phosphates in the perfused normotensive and spontaneously hypertensive rat heart. A 31P NMR study.
复制标题
灌注正常血压和自发性高血压大鼠心脏中的细胞内游离镁和高能磷酸盐。
DOI：
10.1093/ajh/4.2.131
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发表时间：
1991
影响因子：
3.2
通讯作者：
Gupta,RK
中科院分区：
文献类型：
--
作者：
Jelicks,LA;Gupta,RK
我们采用31P核磁共振(31P)波谱技术，以Wistar-京都大鼠(WKY)为对照，以自发性高血压大鼠(SHR)为高血压模型，研究了细胞内游离镁离子([Mg2+]In)、细胞内pH、高能磷酸盐与遗传性高血压的关系。对照组和高血压大鼠(7~12周龄)的平均收缩压(尾套法)分别为113±4 mm Hg(平均±2 SE，n=14)和162±5 mm Hg(平均±2 SE，n=17)。灌流的高血压大鼠心肌细胞内游离镁离子水平(45 2±39µm ol/L，Mean±2 SE，n=17)明显低于对照组(75 6±52µm o l/L，n=14)，而SHR组心肌细胞内pH(7.0 2±0.0 3，Mean±2 SE，n=7)与对照组(7.03±0.0 3，n=7)无明显差异。自发性高血压大鼠心肌细胞内P-肌酸和三磷酸腺苷水平虽无明显差异，但胞内PI值是对照组的2倍(5.71±2.2 8，n=2.92±0.66，n=4)，而磷酸化电势则相应降低(3.0×10 4±1.6×10 4 v 8.3 1.6×10 4×8.3×10 4±1.4=10 4(m ol/L)-1，n=4)；自发性高血压组104±1.4×1.6×104×8.3×104±1.4×104(摩尔/L)-1n=4；自发性高血压组与WKY组比较104(摩尔/L)-1。这些数据显示心肌中游离镁的消耗，并提示高血压患者心脏生物能量学的改变。Am J Hyperten 1991；4：131-136
We have employed31P nuclear magnetic resonance (NMR) spectroscopy to examine the relationship between cytosolic free Mg2++ ([Mg2+]in), intracellular pH, high energy phosphates, and genetic hypertension using the Wistar-Kyoto rat (WKY) as a control and the spontaneously hypertensive rat (SHR) as a model for essential hypertension. The mean systolic blood pressures (measured using the tail cuff method) of control and hypertensive rats (aged 7 to 12 weeks) were 113 ± 4 mm Hg (mean ± 2 SE, n = 14) and 162 ± 5 mm Hg (mean ± 2 SE, n = 17), respectively. Intracellular free Mg2++ levels were significantly depleted in the isolated Langendorff perfused hypertensive rat hearts (452 ± 39µmol/L, mean ± 2 SE, n = 17) compared to control hearts (756 ± 52µmol/L, n = 14); however, intracellular pH did not differ in the SHR hearts (7.02 ± 0.03, mean ± 2 SE,n = 7) compared with controls (7.03 ± 0.03, n = 7). Although we could not demonstrate a statistically significant difference in the levels of P-creatine or ATP, intracellular Piwas two-fold higher (5.71 ± 2.28 mmol/L, n 2.92 ± 0.66 mmol/L, n = 4) and the phosphorylation potential, [MgATP]/[MgADP][Pi], was correspondingly lower (3.0 × 104 ± 1.6 × 104v 8.3 1.6 × 104× 8.3 × 104± 1.4 = 104 (mol/L)-1, n = 4) i n SHR ; 104 ± 1.4 1.6 × 104× 8.3 × 104± 1.4 × 104 (mol/L)-1n = 4) i n SHR ; 104 (mol/L)-1, n = 4) in SHR compared to WKY hearts. These data demonstrate free magnesium depletion in heart muscle and indicate an alteration in cardiac bioenergetics in essential hypertension. Am J Hypertens 1991;4:131–136