Evidence for renotropic activity in ovine pituitaries.
Evidence for renotropic activity in ovine pituitaries.
复制标题
绵羊垂体促肾活性的证据。
作者：
W. Nicholson;R. Barton;D. Puett;D. Orth;G. Liddle
在体重75-150克的完整和切除脑垂体的雄性SD大鼠中，肾脏重量与体重呈线性关系；然而，在完整动物中，给定体重的肾脏重量更大。制备了一种部分纯化的绵羊脑垂体提取物，它具有迄今未被描述的特性，能刺激去垂体动物的肾脏生长，达到类似大小的完整大鼠的预期水平。6天前切除垂体的大鼠，每天注射10次垂体提取物，分析GH、ACTH和LH的含量，肾脏重量显著增加。相反，用与提取物中发现的数量类似的生长激素加ACTH处理的其他垂体切除动物的肾脏大小并没有超过同等大小的垂体切除大鼠的预期。排除了黄体生成素可能通过雄激素产生的间接作用，证明了黄体生成素对小鼠肾脏的生长有相似的作用。
A linear relationship was found for kidney weight vs. body weight in both intact and hypophysectomized male Sprague-Dawley rats, weighing 75–150 g; however, kidney weight was greater for a given body weight in intact animals. A partially purified extract of ovine pituitaries was prepared which had the heretofore undescribed property of stimulating renal growth in hypophysectomized animals to the level expected for intact rats of similar size. When rats, hypophysectomized 6 days previously, were given 10 daily injections of the pituitary extract, which was analyzed for GH, ACTH, and LH content, renal weight increased significantly. In contrast, kidney size in other hypophysectomized animals treated with a quantity of GH plus ACTH similar to that found in the extract did not increase beyond that expected for a hypophysectomized rat of comparable size. A possible indirect action of LH in the extract via androgen production was excluded by demonstrating that the extract produced similar renal growth in both h...