Fabrication of Antimicrobial Multilayered Nanofibrous Scaffolds-Loaded Drug via Electrospinning for Biomedical Application.
Fabrication of Antimicrobial Multilayered Nanofibrous Scaffolds-Loaded Drug via Electrospinning for Biomedical Application.
复制标题
通过静电纺丝为生物医学应用制造抗菌多层纳米纤维支架的药物。
DOI：
10.3389/fbioe.2021.755777
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发表时间：
2021
影响因子：
5.7
通讯作者：
Wu Z
中科院分区：
文献类型：
--
作者：
Liu Q;Jia H;Ouyang W;Mu Y;Wu Z
由生物基材料制备的纳米纤维具有良好的生物相容性、生物可降解性和力学性能，在生物医学领域有着广泛的应用。在此，我们制备了多层纳米纤维支架，以提高药物输送性能。通过多层静电纺丝将疏水性聚乳酸（PLA）：聚己内酯（PCL）和亲水性聚乙烯醇（PVA）纳米纤维复合到复合支架上。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）测量的所开发的支架的形态和结构特征证实了纳米级范围内的平滑和均匀的纤维。单层PVA纳米纤维与多层支架之间的接触角和傅里叶变换红外光谱（FTIR）的差异证实了PLA：PCL表面的存在。体外生物降解和药物释放分析表明，多层支架具有良好的生物降解性和潜在的医疗应用。抑菌圈试验表明，由于模型药物的掺入，支架对大肠杆菌和金黄色葡萄球菌具有良好的抗菌活性。这些结果表明，多层支架被证明是生物医学应用中理想的抗菌材料。
Nanofibers prepared by biobased materials are widely used in the field of biomedicine, owing to outstanding biocompatibility, biodegradable characters, and excellent mechanical behavior. Herein, we fabricated multilayered nanofibrous scaffolds in order to improve the performance of drug delivery. The composite layer-by-layer scaffolds were incorporated by hydrophobic poly(l-lactic acid) (PLA): polycaprolactone (PCL) and hydrophilic poly(vinyl alcohol) (PVA) nanofibers via multilayer electrospinning. Morphological and structural characteristics of the developed scaffolds measured by scanning electron microscopy (SEM), and transmission electron microscopy (TEM) confirmed smooth and uniform fibers ranging in nanometer scale. The differences in contact angles and Fourier transform infrared spectrum (FTIR) between single-layered PVA nanofibers and multilayered scaffolds verified the existence of PLA: PCL surface. In vitro biodegradable and drug release analysis depicted multilayered scaffolds had good biodegradability and potential for medical application. Due to the model drug incorporation, scaffolds exhibited good antibacterial activity against Escherichia coli and Staphylococcus aureus by the zone of inhibition test. These results revealed that the multilayered scaffolds were proved to be desirable antibacterial materials for biomedical application.