RAPID SPECTROPHOTOMETRIC ASSAY FOR CARNITINE PALMITOYLTRANSFERASE
RAPID SPECTROPHOTOMETRIC ASSAY FOR CARNITINE PALMITOYLTRANSFERASE
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DOI：
10.1016/0003-2697(72)90061-9
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发表时间：
1972-01-01
影响因子：
2.9
通讯作者：
HELMRATH, T
中科院分区：
文献类型：
--
作者：
BIEBER, LL;ABRAHAM, T;HELMRATH, T
介绍了肉碱棕榈酰基转移酶（CPT）快速灵敏分光光度法测定的实验条件。用5,5′-二硫代比斯-（2-硝基苯甲酸）（DTNB）测定CPT从棕榈酰辅酶a中释放的还原酶a的量。由于可以通过其他反应（即去酰基化或转酰基化）对棕榈酰辅酶a形成的还原辅酶a的量进行校正，因此该测定可用于不纯的CPT制剂，如肝线粒体。
The experimental conditions for a rapid and sensitive spectrophotometric assay for carnitine palmitoyltransferase (CPT) are described. The amount of reduced CoA liberated from palmitoyl CoA by CPT is quantitated using 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB). Since corrections can be made for the amount of reduced CoA formed from palmitoyl CoA by other reactions, i.e., deacylations or transacylations, the assay can be used with impure CPT preparations such as liver mitochondria.