Application of heated electrodes operating in a non-isothermal mode for interference elimination with amperometric biosensors
Application of heated electrodes operating in a non-isothermal mode for interference elimination with amperometric biosensors
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DOI：
10.1007/s00216-004-2585-2
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发表时间：
2004-05-01
影响因子：
4.3
通讯作者：
Gründler, P
中科院分区：
文献类型：
--
作者：
Lau, C;Reiter, S;Gründler, P
将加热电极应用于基于葡萄糖氧化酶的电流型葡萄糖生物传感器的非等温操作，所述葡萄糖氧化酶通过截留在聚合物层内而固定在电极表面上。的局部沉积的聚合物膜的同时截留的酶是通过电化学诱导的pH值调制在电极前面的扩散区，从而改变的聚合物链的溶解度。该非手动传感器制备方案可成功用于新型间接加热电极的修改。非等温操作模式允许在酶传感器的最佳温度下工作，而不会使本体溶液发生任何热变形。增加表面温度的传感器，从而加速运输以及动力学过程，导致在一个增强的电流信号，在干扰化合物，如抗坏血酸的存在下，所提出的技术允许使用的发散热影响的传感过程中，对于不同的电化学活性化合物，在不同的电极温度下的电流信号的去卷积。本文提出了一种在干扰物存在下测定葡萄糖的计算方法，作为计算消除干扰的基础。
Heated electrodes were applied for the non-isothermal operation of amperometric glucose biosensors based on glucose oxidase immobilised on the electrode surface by entrapment within a polymer layer. The localised deposition of the polymer film under simultaneous entrapment of the enzyme was achieved by an electrochemically induced pH-modulation in the diffusion zone in front of the electrode, thus altering the solubility of the polymer chains. This non-manual sensor preparation protocol could be successfully used for the modification of a novel indirectly heated electrode. The non-isothermal operating mode allows working at the optimum temperature of the enzyme sensors without any thermal distortion of the bulk solution. Increased surface temperature of the sensor thus accelerates transport as well as kinetic processes, resulting in an enhanced amperometric signal.In the presence of interfering compounds such as ascorbic acid, the proposed technique allows use of the diverging thermal impact on the sensing process, for different electrochemically active compounds, for a deconvolution of the amperometric signal at different electrode temperatures. A calculation method for determination of glucose in the presence of one interfering compound is presented as a basis for a calculative interference elimination.