Development and characterisation of Brassica napus-Sinapis arvensis addition lines exhibiting resistance to Leptosphaeria maculans
Development and characterisation of Brassica napus-Sinapis arvensis addition lines exhibiting resistance to Leptosphaeria maculans
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DOI：
10.1007/s001220051574
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发表时间：
2000-11-01
影响因子：
5.4
通讯作者：
Sacristán, MD
中科院分区：
文献类型：
--
作者：
Snowdon, RJ;Winter, H;Sacristán, MD
由黄斑细螺旋体引起的油菜黑腿病是世界范围内影响油菜的主要病害之一。Sinapis arvensis对黑腿菌对油菜（芸苔）抗性的转移是有价值的，因为该物种对各种侵袭性黑腿菌分离株具有很高的抗性。其中包括至少一种澳大利亚分离物，该分离物已被发现克服了来自具有芸苔B基因组的物种的抗性，直到现在，这种抗性是黑腿抗性种间转移的主要来源。采用基因组原位杂交（GISH）技术对甘蓝型油菜与葡萄树属间杂交的回交后代进行了植物病理学研究和分子细胞遗传学分析。BC3S的后代包括高幼苗（子叶）的可育植株和成年植株的抗性，这些抗性与葡萄的顶心附加染色体的存在有关。此外，一些具有成体抗性但子叶敏感的个体核型正常，没有明显的GISH信号，表明可能存在抗性渗入系。对3株BC3高抗性植物的自交后代的植物病理学分析表明，幼苗和成株的抗性可能是由不同的基因座赋予的。
Blackleg caused by Leptosphaeria maculans is one of the most important diseases affecting oilseed rape worldwide. Sinapis arvensis is valuable for the transfer of blackleg resistance to oilseed rape (Brassica napus) because this species contains high resistance against various aggressive isolates of the blackleg fungus. These include at least one Australian isolate which has been found to overcome resistance originating from species with the Brassica B genome, until now the major source for interspecific transfer of blackleg resistance. Backcross offspring from intergeneric crosses between Brassica napus and S. arvensis were subjected to phytopathological studies and molecular cytogenetic analysis with genomic in situ hybridisation (GISH). The BC3S progenies included fertile plants exhibiting high seedling (cotyledon) and adult plant resistance associated with the presence of an acrocentric addition chromosome from S. arvensis. In addition, some individuals with adult plant resistance but cotyledon susceptibility were observed to have a normal B. napus karyotype with no visible GISH signals, indicating possible resistant introgression lines. Phytopathological analysis of selfing progenies from 3 different highly resistant BC3 plants showed that seedling and adult plant resistance are probably conferred by different loci.