Regulated expression of endothelial lipase in atherosclerosis
Regulated expression of endothelial lipase in atherosclerosis
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DOI：
10.1016/j.mce.2009.11.003
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发表时间：
2010-02-05
影响因子：
4.1
通讯作者：
Liu, Peiqing
中科院分区：
文献类型：
--
作者：
Wu, Xiaoqian;Huang, Heqing;Liu, Peiqing
内皮脂酶（EL）是高密度脂蛋白（HDL）代谢的主要决定因素，与动脉粥样硬化的发生发展有关，但EL在动脉粥样硬化中的表达调控尚不清楚。本研究旨在探讨脂多糖（LPS）对大鼠动脉粥样硬化Raw 264.7细胞EL表达的影响及其可能的作用机制。采用高胆固醇饲料（HCD）联合维生素D-2（VD）建立大鼠动脉粥样硬化模型。免疫组化和Western blotting结果显示，EL在HCD大鼠主动脉中的表达增强，尤其是在粥样硬化病变部位。LPS可诱导Raw 264.7细胞EL表达，并呈时间和剂量依赖性，NF κ B B抑制剂PDTC可减弱LPS对EL表达的影响。EMSA显示LPS可刺激NF κ B B与EL启动子结合。总之，EL在大鼠动脉粥样硬化中上调，并且LIPS通过NF κ B活化在体外刺激EL表达。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Endothelial lipase (EL) is a major determinant of HDL metabolism and associated with the development of atherosclerosis, however the regulated expression of EL in atherosclerosis is unclear. In this study, we investigated EL expression in rat atherosclerosis and explored the potential mechanisms regulating EL expression by employing LPS on Raw264.7 cells in vitro. Rat atherosclerosis model was established fed on high-cholesterol diet (HCD) combined with vitamin D-2 (VD). Western blotting and immunochemistry staining revealed that EL expression was increased in the aorta, especially the atherosclerotic lesions in HCD rats. LPS increased EL expression in a time and dose dependent manner in Raw264.7 cells and NF kappa B inhibitor, PDTC attenuated the effects of LPS on EL EMSA revealed that LPS stimulated NF kappa B binding to EL promoter. In summary, EL was upregulated in rat atherosclerosis and LIPS stimulates EL expression in vitro through NF kappa B activation. (C) 2009 Elsevier Ireland Ltd. All rights reserved.