Hypertonic saline treatment of acid aspiration-induced lung injury
Hypertonic saline treatment of acid aspiration-induced lung injury
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DOI：
10.1006/jsre.1996.0028
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发表时间：
1996-01-01
影响因子：
2.2
通讯作者：
Neville, LF
中科院分区：
文献类型：
--
作者：
Rabinovici, R;Vernick, J;Neville, LF
气道酸吸入导致严重的肺微血管损伤和肺水肿。最近的研究表明，与微血管损伤相关的其他疾病，如败血症和烧伤，可以有效地治疗低容量高渗盐水（HTS）。因此，本研究的目的是测试高温超导是否减弱吸入诱导的肺损伤的大鼠。腹腔注射0.2ml 0.1NHCl（n = 7）可引起肺白细胞隔离[髓过氧化物酶（MPO）活性+446 +/-34%，P < 0.05;支气管肺泡灌洗液（BAL）中性粒细胞计数+1.78 +/-23%，P < 0.05]，水肿（+43 +/-6%，P < 0.01）和微血管通透性缺陷（BAL蛋白浓度+675 +/-34%，P < 0.01）。这些变化与组织缺氧（骨骼肌PO 2，49 +/- 8 mm Hg，P < 0.05）和血清TNF α升高（750 +/- 38 pg/ml，P < 0.01）有关。HTS（2400 mosmole/L）5 ml/kg，抽吸后20 min给药（n = 7），肺水肿减轻58 +/- 7%（P < 0.05），组织氧分压改善（PO 2，85 +/- 7 mm Hg，P < 0.05），但未能改变肺MPO和BAL液蛋白和白细胞计数反应。此外，HTS没有降低TNF α对抽吸的反应。这些数据表明，低容量HTS在治疗吸入性肺损伤中具有潜在的治疗作用。此外，我们的数据表明，HTS是通过快速转移液体从肺动脉到血管内室，因为它没有抑制炎症反应的吸入。(C)出版社：Academic Press，Inc.
Airway acid aspiration leads to severe microvascular lung injury and pulmonary edema. Recent studies have demonstrated that other conditions associated with microvascular injury such as sepsis and burns can be effectively treated with low-volume hypertonic saline (HTS). Thus, the present study aimed to test whether HTS attenuates aspiration-induced lung injury in the rat. Intratracheal administration of 0.2 mi of 0.1 N HCl (n = 7) induced pulmonary leukosequestration [myeloperoxidase (MPO) activity +446 +/- 34%, P < 0.05; bronchoalveolar lavage (BAL) fluid neutrophil count +1.78 +/- 23%, P < 0.05], edema (+43 +/- 6%, P < 0.01), and microvascular permeability defect (BAL protein concentration +675 +/- 34%, P < 0.01). These changes were associated with tissue hypoxia (skeletal muscle PO2, 49 +/- 8 mm Hg, P < 0.05) and elevated serum TNF alpha (750 +/- 38 pg/ml, P < 0.01). HTS (2400 mosmole/liter) at 5 ml/kg, administered 20 min after aspiration (n = 7), reduced lung pulmonary edema by 58 +/- 7% (P < 0.05) and improved tissue oxygen tension (PO2, 85 +/- 7 mm Hg, P < 0.05) but failed to alter lung MPO and BAL fluid protein and leukocyte count response. Also, HTS did not reduce TNF alpha response to aspiration. These data point to a potential therapeutic role for low-volume HTS in treating aspiration-induced lung injury. In addition, our data suggest that HTS is acting by rapidly shifting fluid from the pulmonary interstitium to the intravascular compartment because it did not inhibit the inflammatory response to aspiration. (C) 1996 Academic Press, Inc.