Nondestructive Characterization of Stem Cell Neurogenesis by a Magneto-Plasmonic Nanomaterial-Based Exosomal miRNA Detection
Nondestructive Characterization of Stem Cell Neurogenesis by a Magneto-Plasmonic Nanomaterial-Based Exosomal miRNA Detection
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DOI：
10.1021/acsnano.9b01875
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发表时间：
2019-08-01
期刊：
影响因子：
17.1
通讯作者：
Lee, Ki-Bum
中科院分区：
文献类型：
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作者：
Lee, Jin-Ho;Choi, Jin-Ha;Lee, Ki-Bum
干细胞治疗神经退行性疾病的全面实现需要对干细胞命运的精确控制和表征。在此，我们报告了一个多功能磁等离子体纳米棒（NR）检测平台，以解决当前干细胞神经发生破坏性表征方法的局限性。外泌体及其内部内容物在细胞相互作用和细胞内在调控中起着重要作用，作为下一代生物标志物受到广泛关注。此外，外泌体microRNAs （miRNA）也为细胞质成分的非破坏性分子分析提供了重要途径。为此，我们开发的非破坏性，选择性和敏感的检测平台具有(i)用于外泌体分离的免疫磁性活性成分和（ii）用于敏感外泌体miRNA检测的等离子体/金属增强荧光成分，以表征干细胞分化。在概念验证演示中，我们的多功能磁等离子体NR成功检测了miRNA-124的表达水平，并以一种无损和有效的方式表征了人类诱导的多能干细胞衍生的神经干细胞的神经发生。此外，我们通过在离体啮齿动物模型中表征异质神经细胞群，证明了多功能磁等离子体核磁共振的多功能性和可行性。总之，我们相信我们基于磁等离子体nr的多功能外泌体miRNA检测平台在研究细胞相互作用和控制干细胞分化的内在细胞调节因子的功能方面具有很大的潜力。
The full realization of stem cell-based treatments for neurodegenerative diseases requires precise control and characterization of stem cell fate. Herein, we report a multifunctional magneto-plasmonic nanorod (NR)-based detection platform to address the limitations associated with the current destructive characterization methods of stem cell neurogenesis. Exosomes and their inner contents have been discovered to play critical roles in cell cell interactions and intrinsic cellular regulations and have received wide attention as next-generation biomarkers. Moreover, exosomal microRNAs (miRNA) also offer an essential avenue for nondestructive molecular analyses of cell cytoplasm components. To this end, our developed non-destructive, selective, and sensitive detection platform has (i) an immunomagnetic active component for exosome isolation and (ii) a plasmonic/metal-enhanced fluorescence component for sensitive exosomal miRNA detection to characterize stem cell differentiation. In a proof-of-concept demonstration, our multifunctional magneto-plasmonic NR successfully detected the expression level of miRNA-124 and characterized neurogenesis of human-induced pluripotent stem cell-derived neural stem cells in a nondestructive and efficient manner. Furthermore, we demonstrated the versatility and feasibility of our multifunctional magneto-plasmonic NRs by characterizing a heterogeneous population of neural cells in an ex vivo rodent model. Collectively, we believe our multifunctional magneto-plasmonic NR-based exosomal miRNA detection platform has a great potential to investigate the function of cell cell interactions and intrinsic cellular regulators for controlling stem cell differentiation.