Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production
Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production
复制标题
DOI：
10.1007/s11274-008-9933-x
复制
发表时间：
2009-04-01
影响因子：
4.1
通讯作者：
Lumyong, Saisamorn
中科院分区：
文献类型：
--
作者：
Khamna, Sutthinan;Yokota, Akira;Lumyong, Saisamorn
从16种药用植物根际土壤中分离到放线菌445株。形态和化学分类研究表明，89%的菌株属于链霉菌属，11%的菌株为非链霉菌：放线菌属、小双孢菌属、小单孢菌属、诺卡氏菌属、非链霉菌属。另有3个菌株未分类。从姜黄根际土壤中分离到的放线菌数量最多，多样性最高。23株链霉菌对5种植物病原真菌中的至少一种表现出抑制活性：油菜链格孢菌、球孢链霉菌、尖孢镰刀菌、指状青霉和罗氏菌核。结果表明，36株放线菌具有产生吲哚-3-乙酸的能力，75株在铬天青S(CAS)琼脂上产生铁载体。链霉菌CMU-PA101和链霉菌CMU-SK126产生抗真菌化合物、IAA和铁载体的能力较强。
A total of 445 actinomycete isolates were obtained from 16 medicinal plant rhizosphere soils. Morphological and chemotaxonomic studies indicated that 89% of the isolates belonged to the genus Streptomyces, 11% were non-Streptomycetes: Actinomadura sp., Microbispora sp., Micromonospora sp., Nocardia sp, Nonomurea sp. and three isolates were unclassified. The highest number and diversity of actinomycetes were isolated from Curcuma mangga rhizosphere soil. Twenty-three Streptomyces isolates showed activity against at least one of the five phytopathogenic fungi: Alternaria brassicicola, Collectotrichum gloeosporioides, Fusarium oxysporum, Penicillium digitatum and Sclerotium rolfsii. Thirty-six actinomycete isolates showed abilities to produce indole-3-acetic acid (IAA) and 75 isolates produced siderophores on chrome azurol S (CAS) agar. Streptomyces CMU-PA101 and Streptomyces CMU-SK126 had high ability to produced antifungal compounds, IAA and siderophores.