Dynamic expression of intra- and extra-cellular proteome and the influence of epiphytic bacteria for Nostoc flagelliforme in response to rehydration
Dynamic expression of intra- and extra-cellular proteome and the influence of epiphytic bacteria for Nostoc flagelliforme in response to rehydration
复制标题
发菜细胞内外蛋白质组的动态表达及附生细菌对补水反应的影响
DOI：
10.1111/1462-2920.14931
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发表时间：
2020
影响因子：
5.1
通讯作者：
Wan Cuihong
中科院分区：
文献类型：
--
作者：
Wang Bing;Yang Jingjing;Xu Chen;Yi Lanxing;Wan Cuihong
发菜（Nostoc flagelliformme）是荒漠生物结皮中生态适应性较强的一种。然而，其恢复从静止到活跃状态后，长期休眠的机制仍然很差的特点。特别是胞外蛋白质组如何与适应策略相关，参与微藻-细菌的相互作用。在目前的工作中，我们首次分析了在灭菌和自然条件下完整的复水期内的鞭毛藻细胞内和细胞外蛋白质组。发状微囊藻蛋白质表达谱在湿润后的前1 h波动较大，而在完全水合后相对稳定。根据胞外蛋白质组数据集，我们发现了各种胞外水解酶和膜转运蛋白的动态分泌，它们分别与肽聚糖消化和营养交换有关。灭菌诱导的213个差异表达蛋白也反映了营养交换的变化，并突出了发状微囊藻与周围细菌之间的共生关系。我们还鉴定了95蛋白的112个磷酸肽和217个磷酸化位点。我们在这里提出的时间过程数据集将是一个参考，了解N. flagelliformere复苏及其在微生物群落多样性和土壤荒漠化控制的潜在作用的分子过程。
Nostoc flagelliformeis well known for its strong ecological adaptability in inhabiting desert biological soil crusts. However, the mechanism of its recovery from quiescent to active state after prolonged dormancy remains poorly characterized. Especially how exoproteome be related to the adaptive strategies and participate in the microalgae‐bacteria interaction. In the present work, we analysed the intra‐ and extra‐cellular proteome ofN.flagelliformeover a complete rehydration period both in sterilization and in natural condition for the first time. The protein expression profile forN.flagelliformehas more fluctuations during the first 1 h after wetting but been relatively steady after fully hydrated. According to the extracellular proteomic datasets, we found a dynamic secretion of various extracellular hydrolytic enzymes and membrane transport proteins, which were related to peptidoglycan digestion and nutrient exchange respectively. Two‐hundred and thirteen differentially expressed proteins induced by sterilization also reflect variation in nutrient exchange and highlight symbiosis betweenN.flagelliformeand surrounding bacteria. We also identified 112 phosphopeptides and 217 phosphorylation site of 95 protein of hydratedN.flagelliforme. The time course datasets we present here will be a reference for understanding the molecular processes underlyingN.flagelliformeresuscitation and its potential role in microbial community diversification and soil desertification control.