The effect of neuraminidase on the relative surface charge-associated properties of rat red blood cells of different ages.
The effect of neuraminidase on the relative surface charge-associated properties of rat red blood cells of different ages.
复制标题
神经氨酸酶对不同年龄大鼠红细胞相对表面电荷相关特性的影响。
DOI：
10.1016/0005-2736(83)90137-2
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Seaman,GV
中科院分区：
文献类型：
--
作者：
Walter,H;Tamblyn,CH;Krob,EJ;Seaman,GV
约大鼠红细胞表面70%的唾液酸易被神经氨酸酶（霍乱弧菌）裂解。神经氨酸酶处理导致红细胞在带电葡聚糖-聚（乙二醇）水相系统中的分配系数（K）和细胞的电泳迁移率降低。大鼠神经氨酸酶处理的红细胞，含有不同年龄的59 Fe标记的成熟红细胞的逆流分布表明：（a）红细胞在不同腔沿着提取列的电泳迁移率随K的增加而增加，与未处理的红细胞的情况一样，和（B）细胞年龄相关的表面电荷相关性质的差异既不消除，也不改变酶的作用。
Approx. 70% of the sialic acid on the rat erythrocyte surface is susceptible to cleavage by neuraminidase (Vibrio cholerae). Neuraminidase treatment results in a reduction in the partition coefficient (K) of the red cells in a charged dextran-poly(ethylene glycol) aqueous phase system and in the electrophoretic mobility of the cells. Countercurrent distribution of rat neuraminidase-treated erythrocytes, containing59Fe-labeled mature red cells of distinct age, indicates that (a) the electrophoretic mobilities of red cells in different cavities along the extraction train increase with increasingK, as is the case with untreated erythrocytes, and (b) the cell age-related differences in surface charge-associated properties are neither eliminated nor altered by the enzyme action.