Gastric adenocarcinoma microRNA profiles in fixed tissue and in plasma reveal cancer-associated and Epstein-Barr virus-related expression patterns.
Gastric adenocarcinoma microRNA profiles in fixed tissue and in plasma reveal cancer-associated and Epstein-Barr virus-related expression patterns.
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DOI：
10.1038/labinvest.2016.33
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Gulley ML
中科院分区：
文献类型：
--
作者：
Treece AL;Duncan DL;Tang W;Elmore S;Morgan DR;Dominguez RL;Speck O;Meyers MO;Gulley ML
福尔马林固定石蜡包埋组织（FFPE）或血浆中的microRNA表达可能为癌症管理增加价值。GastroGenus miR Panel被开发用于测量55种癌症特异性人类microRNA、EB病毒（EBV）编码的microRNA和对照。将该Q-rtPCR组应用于100个富集腺癌或邻近非恶性粘膜的FFPE和31名患者的血浆。在FFPE中，恶性胃粘膜与邻近良性胃粘膜中上调的microRNA为hsa-miR-21、-155、-196 a、-196 b、-185和-let-7i。Hsa-miR-18 a、34 a、187、-200a、-423-3p、-484和-744下调。癌症与非癌症对照的血浆具有上调的hsa-miR-23 a、-103和-221和下调的hsa-miR-378、-346、-486-5p、-200b、-196a、-141和-484。EBV感染与未感染的癌症表达多种EBV编码的microRNA，并且伴随的四种人类microRNA的失调表明病毒感染可能改变细胞生化途径。人microRNA在恶性和良性胃粘膜之间以及癌症患者和非癌症对照之间的血浆中失调。EBV microRNA表达与已知EBV状态的强相关性强调了microRNA技术反映疾病生物学的能力。与独特的人类microRNA一致的病毒microRNA的表达为病毒肿瘤发生提供了新的见解，并加强了microRNA谱的潜力，以帮助胃癌亚型的分类。对血浆的初步研究表明，它有可能成为癌症诊断的非侵入性补充。
MicroRNA expression in formalin fixed paraffin embedded tissue (FFPE) or plasma may add value for cancer management. The GastroGenus miR Panel was developed to measure 55 cancer-specific human microRNAs, Epstein-Barr virus (EBV)-encoded microRNAs, and controls. This Q-rtPCR panel was applied to 100 FFPEs enriched for adenocarcinoma or adjacent non-malignant mucosa, and to plasma of 31 patients. In FFPE, microRNAs upregulated in malignant versus adjacent benign gastric mucosa were hsa-miR-21, -155, -196a, -196b, -185, and -let-7i. Hsa-miR-18a, 34a, 187, -200a, -423-3p, -484 and -744 were downregulated. Plasma of cancer versus non-cancer controls had upregulated hsa-miR-23a, -103 and -221 and downregulated hsa-miR-378, -346, -486-5p, -200b, -196a, -141 and -484. EBV infected versus uninfected cancers expressed multiple EBV-encoded microRNAs, and concomitant dysregulation of four human microRNAs suggests that viral infection may alter cellular biochemical pathways. Human microRNAs were dysregulated between malignant and benign gastric mucosa and between plasma of cancer patients and non-cancer controls. Strong association of EBV microRNA expression with known EBV status underscores the ability of microRNA technology to reflect disease biology. Expression of viral microRNAs in concert with unique human microRNAs provides novel insights into viral oncogenesis and reinforces the potential for microRNA profiles to aid in classifying gastric cancer subtypes. Pilot studies of plasma suggest the potential for a non-invasive addition to cancer diagnostics.
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影响因子：
6.4
作者：
Destro, Federica;Sforza, Fabio;Gavioli, Riccardo
通讯作者：
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影响因子：
5.4
作者：
Feng, WH;Hong, G;Kenney, SC
通讯作者：
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DOI：
10.1111/j.1440-1746.2008.05666.x
发表时间：
2009-04-01
影响因子：
4.1
作者：
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作者：
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通讯作者：
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影响因子：
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作者：
Feng, WH;Cohen, JI;Kenney, SC
通讯作者：
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