INVOLVEMENT OF THE BCL-2 GENE IN HUMAN FOLLICULAR LYMPHOMA
INVOLVEMENT OF THE BCL-2 GENE IN HUMAN FOLLICULAR LYMPHOMA
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DOI：
10.1126/science.3874430
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
CROCE, CM
中科院分区：
文献类型：
--
作者：
TSUJIMOTO, Y;COSSMAN, J;CROCE, CM
在B细胞型急性淋巴细胞白血病患者的细胞中，克隆了18号染色体断裂点两侧区域的重组DNA探针;该细胞系的细胞携带t（14;18）染色体易位。两个探针检测到. apprx中的DNA重排。60%的滤泡性淋巴瘤的情况下筛选。在滤泡性淋巴瘤中，染色体18的带q21中的大多数断裂点聚集在DNA的短延伸内，2.1长度上的长度。断裂点两侧区域的18号染色体特异性DNA探针也在各种细胞类型中检测到长度为6个内切酶的RNA转录物。在大多数携带t（14;18）染色体易位的滤泡性淋巴瘤病例中，编码这些转录本的基因（bcl-2基因）似乎被中断。
Recombinant DNA probes were cloned for the areas flanking the breakpoint on chromosome 18 in cells from a patient with acute lymphocytic leukemia of the B-cell type; cells of this line carry the t(14;18) chromosomal translocation. Two of the probes detected DNA rearrangements in .apprx. 60% of the cases of follicular lymphoma screened. In follicular lymphoma, most of the breakpoints in band q21 of chromosome 18 were clustered within a short stretch of DNA, .apprx. 2.1 kilobases in length. Chromosomes 18-specific DNA probes for the areas flanking the breakpoints also detected RNA transcripts 6 kilobases in length in various cell types. The gene coding for these transcripts (the bcl-2 gene) seems to be interrupted in most cases of follicular lymphomas carrying the t(14;18) chromosomal translocation.