Comparative Evaluation of the Seegene Seeplex RV15 and Real-Time PCR for Respiratory Virus Detection
Comparative Evaluation of the Seegene Seeplex RV15 and Real-Time PCR for Respiratory Virus Detection
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DOI：
10.1002/jmv.22125
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发表时间：
2011-08-01
影响因子：
12.7
通讯作者：
Clark, Duncan A.
中科院分区：
文献类型：
--
作者：
Bibby, David F.;McElarney, Iain;Clark, Duncan A.
呼吸道病毒感染在很大程度上导致幼儿住院，并导致免疫功能低下患者（例如患有血液恶性肿瘤的患者）发病。他们的快速而准确的诊断对于患者管理至关重要。为了评估 Seeplex (R) DPO 技术在呼吸道病毒诊断中的预期效用，通过 Seegene Seeplex (R) RV12 系统对一组 99 个呼吸道样本（通过实时 RTPCR 检测出一种或多种病毒呈阳性）进行了分析。 RV12不仅能够检测实时RT-PCR系统中除肠道病毒外的所有10种病毒，还可以区分RSV的两个亚群并检测冠状病毒的两个亚群。 RV12 能够可靠地检测到与 RT-PCR 共有的 9 种病毒中的 7 种。 RV12 未检测到 11 个偏肺病毒 RTPCR 阳性样本和 5 个乙型流感阳性样本。 Seegene 开发了第二代系统 RV15，它不仅可以检测另外三种病毒，还解决了 RV12 特异性的潜在问题。为了解决这些问题，我们使用 RV15 对 84 份通过实时 PCR 检测出一系列病毒呈阳性的呼吸道样本进行了检测。该评估结果比 RV12 的结果显着改善。 Seeplex (R) 系统提供的高通量能力和潜在的较低技术要求可能会成为实时 RT-PCR 系统的替代方案。 J. Med。病毒。 83:1469-1475, 2011。(C) 2011 Wiley-Liss, Inc.
Respiratory virus infections contribute substantially both to hospitalizations of young children, and to morbidity in immunocompromised patients such as those with hematological malignancies. Their rapid and accurate diagnosis is essential to patient management. To evaluate the prospective utility of Seeplex (R) DPO technology in respiratory virus diagnosis, a panel of 99 respiratory samples positive by real-time RTPCR for one or more viruses was assayed by the Seegene Seeplex (R) RV12 system. As well as being able to detect all 10 viruses in the real-time RT-PCR system with the exception of enteroviruses, RV12 can also distinguish between the two subgroups of RSV and detect two subgroups of coronaviruses. Seven of the nine viruses in common with the RT-PCR were detected reliably by RV12. Eleven samples RTPCR-positive for Metapneumovirus and five samples positive for influenza B were not detected by RV12. Seegene developed a second-generation system, RV15, which not only allowed detection of three additional viruses, but also addressed the potential problems with RV12 specificity. To address these concerns, 84 respiratory samples positive for a range of viruses by real-time PCR were assayed with RV15. The results of this evaluation improved significantly upon those seen with RV12. The high throughput capabilities and potential lower technical requirements afforded by the Seeplex (R) system may offer an alternative to real-time RT-PCR systems. J. Med. Virol. 83:1469-1475, 2011. (C) 2011 Wiley-Liss, Inc.