Production and characterization of biosurfactants from Bacillus licheniformis F2.2

Production and characterization of biosurfactants from Bacillus licheniformis F2.2
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DOI:
10.1271/bbb.67.1239
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发表时间:
2003-06-01
影响因子:
1.6
通讯作者:
Kanaya, S
Kanaya, S
中科院分区:
工程技术4区
文献类型:
--
作者:
Thaniyavarn, J;Roongsawang, N;Kanaya, S

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从泰国的一种发酵食品中分离到一株生物表面活性剂产生菌地衣芽孢杆菌F2.2。该菌株能够产生一种新的生物表面活性剂BL 1193,以及两种流行的脂肽生物表面活性剂plipastatin和surfactin。质谱和FT-IR分析表明,BL 1, 193的分子量为1,193 Da,分子中没有肽部分。当plipastatin和surfactin在营养YPD培养基中大量产生时,BL 1193仅在不含氨基酸的合成DF培养基中产生。根据驱油活性测试,BL 1193的比活性(6.53kBS单位/mg)与表面活性素的比活性(5.78- 6.83kBS单位/mg)相当。
A biosurfactant-producing strain, Bacillus licheniformis F2.2, was isolated from a fermented food in Thailand. The strain was capable of producing a new biosurfactant, BL1193, as well as two kinds of popular lipopeptide biosurfactants, plipastatin and surfactin. Mass spectrometry and FT-IR analysis indicated that BL1193 had a molecular mass of 1,193 Da with no peptide portion in the molecule. While plipastatin and surfactin were abundantly produced in a nutrient YPD medium, BL1193 was produced only in a synthetic DF medium containing no amino acids. According to an oil displacement activity test, the specific activity of BL1193 (6.53 kBS units/mg) is equivalent to that of surfactin (5.78-6.83 kBS units/mg).