COOMASSIE BRILLIANT BLUE STAINING OF LIPIDS ON THIN-LAYER PLATES
COOMASSIE BRILLIANT BLUE STAINING OF LIPIDS ON THIN-LAYER PLATES
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DOI：
10.1016/0003-2697(84)90484-6
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发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
HANDA, S
中科院分区：
文献类型：
--
作者：
NAKAMURA, K;HANDA, S
描述了硅胶TLC板中脂质的考马斯亮蓝染色。这种染色剂被证明是有用的简单和复杂的脂质在TLC板上的宽范围检测。它可以染色几类脂质，包括胆固醇、胆固醇酯、甘油酯、磷脂、神经酰胺以及中性和酸性鞘糖脂。它可以均匀地染色斑点，无需腐蚀性试剂，使用简单，适合储存。该染色剂对脂质的视觉检测极限为0.05-0.5 μ g。
Coomassie Brilliant Blue staining of lipids in silica gel TLC plates is described. This stain proved to be useful for the wide-range detection of simple and complex lipids on TLC plates. It can stain several classes of lipids, including cholesterol, cholesterol esters, glycerides, phospholipids, ceramides and neutral and acidic glycosphingolipids. It stains the spots evenly without a corrosive reagent, and is simple to use and suitable for storage. The visual detection limits of this stain for lipids were 0.05-0.5 .mu.g.