Antiviral compounds show enhanced activity in HIV-1 single cycle pseudovirus assays as compared to classical PBMC assays
Antiviral compounds show enhanced activity in HIV-1 single cycle pseudovirus assays as compared to classical PBMC assays
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DOI：
10.1016/j.jviromet.2007.11.009
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发表时间：
2008-03-01
影响因子：
3.1
通讯作者：
Vanham, Guido
中科院分区：
文献类型：
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作者：
Heyndrickx, Leo;Vermoesen, Tine;Vanham, Guido
HIV-1 Env假型病毒（PV）是研究干扰病毒进入靶细胞的化合物的抗病毒活性的有吸引力的工具。为了研究PV试验中获得的结果是否具有生物学相关性，使用三种试验对不同进化枝的5种病毒分离株比较了6种参比化合物的抗病毒活性：（1）外周血单核细胞（PBMCs）中复制病毒，（2）CD 4中的PV和CCR 5-或CXCR 4共受体表达的幽灵细胞，和（3）PBMCs中的PV。两种单循环PV检测方法之间存在显著的线性关系（P <0.0001，R-2 = 0.75）。此外，与使用复制能力病毒的PBMC检测相比，两种检测均显示出对检测的抗逆转录病毒药物的敏感性增强（分别为P=0.013和0.015）。最重要的是，后一种检测的结果可以从两种PV检测中显著预测，其中Ghost靶细胞（P
HIV-1 Env pseudotyped viruses (PV) are an attractive tool for studying the antiviral activities of compounds interfering with virus entry into a target cell. To investigate whether results obtained in PV assays are relevant biologically, the antiviral activity of 6 reference compounds was compared on 5 virus isolates of different clades using three assays: (1) replicating virus in peripheral blood mononuclear cells (PBMCs), (2) PV in CD4 and CCR5- or CXCR4 co-receptor expressing Ghost cells, and (3) PV in PBMCs. A significant linear relationship was found between both single-cycle PV assays (P < 0.000 1, R-2 = 0.75). Moreover, both assays showed enhanced sensitivity to the antiretrovirals tested (P=0.013 and 0.015, respectively) as compared to the PBMC assay with replication-competent virus. Most importantly, results from the latter assay could be predicted significantly from both PV assays, in which either Ghost target cells (P