The fusing ability of sperm is bestowed by CD9-containing vesicles released from eggs in mice
The fusing ability of sperm is bestowed by CD9-containing vesicles released from eggs in mice
复制标题
DOI：
10.1073/pnas.0710608105
复制
发表时间：
2008-09-02
影响因子：
11.1
通讯作者：
Umezawa, Akihiro
中科院分区：
文献类型：
--
作者：
Miyado, Kenji;Yoshida, Keiichi;Umezawa, Akihiro
在受精过程中，两个性细胞——精子和卵子的细胞核相遇时，膜融合是必不可少的一步。然而，除了CD9和Izumo两种分子的参与外，融合的机制尚不清楚。在这里，我们发现精子-卵子融合是由含有CD9的囊泡介导的，CD9从卵子中释放出来并与精子相互作用。我们证明，具有缺陷精子融合能力的CD9（-/-）卵子会损害含有CD9的小泡的释放。我们通过检测精子与CD9（-/-）卵子在外源含CD9囊泡的融合来研究含CD9囊泡的融合促进活性。此外，通过检测精子与CD9（-/-）卵子的融合，我们发现仓鼠卵子与小鼠卵子具有相似的融合能力。从未受精卵中释放的含有cd9的囊泡为与精子融合所需的机制提供了洞见。
Membrane fusion is an essential step in the encounter of two nuclei from sex cells-sperm and egg-in fertilization. However, aside from the involvement of two molecules, CD9 and Izumo, the mechanism of fusion remains unclear. Here, we show that sperm-egg fusion is mediated by vesicles containing CD9 that are released from the egg and interact with sperm. We demonstrate that the CD9(-/-) eggs, which have a defective sperm-fusing ability, have impaired release of CD9-containing vesicles. We investigate the fusion-facilitating activity of CD9-containing vesicles by examining the fusion of sperm to CD9(-/-) eggs with the aid of exogenous CD9-containing vesicles. Moreover, we show, by examining the fusion of sperm to CD9(-/-) eggs, that hamster eggs have a similar fusing ability as mouse eggs. The CD9-containing vesicle release from unfertilized eggs provides insight into the mechanism required for fusion with sperm.