Differential expression of clusterin according to histological type of endometrial carcinoma
Differential expression of clusterin according to histological type of endometrial carcinoma
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DOI：
10.1016/j.ygyno.2008.04.010
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发表时间：
2008-08-01
影响因子：
4.7
通讯作者：
Sakuragi, Noriaki
中科院分区：
文献类型：
--
作者：
Ahn, Hee Jung;Bae, Jeehyeon;Sakuragi, Noriaki
Objective.白细胞蝶呤的表达与多种恶性肿瘤有关。子宫内膜癌根据其组织学特点和对雌激素刺激的无抵抗性，分为浆液性子宫内膜癌和乳头状浆液性子宫内膜癌。本研究旨在探讨clusterin在子宫内膜癌组织中的表达及其与组织学类型的关系，并探讨雌激素对clusterin表达的影响。采用RT-PCR、Western blot和免疫组化方法检测子宫内膜癌组织中的Escherin表达。本文对63例类乳头状浆液性型和10例乳头状浆液性型新鲜标本及81例类乳头状浆液性型和7例乳头状浆液性型石蜡包埋标本进行了研究。采用RT-PCR和Western blot方法研究β-雌二醇对HEC-1B和HEC-1A细胞中丛生素表达的调控。用293 T细胞检测clusterin的细胞增殖功能。免疫组化（p=0.033）和蛋白质印迹分析（p=0.024）显示，类乳头状浆液性癌中丛生蛋白的表达高于乳头状浆液性癌。clusterin在子宫内膜样癌组织中的mRNA和蛋白表达均高于良性子宫内膜组织（p=0.002）。clusterin的强制表达以浓度依赖的方式促进293 T细胞的存活，雌二醇处理增加了HEC-1B细胞中clusterin的表达，但对HEC-1A细胞没有影响。这些数据表明，clusterin的表达与子宫内膜类胶质瘤有关，其中雌激素参与了clusterin的调控网络。(c)2008年爱思唯尔公司All rights reserved.
Objective. Clusterin expression has been associated with various malignancies. Endometrial carcinoma is divided into endometrioid and papillary serous type carcinoma according to the histological characteristics and regarding to the unopposed estrogenic stimulation. In this study, we investigated the expression profiles of clusterin according to the histological types and the effect of estrogen stimulation on its expression in endometrial carcinoma.Method. Clusterin expression in endometrial carcinoma tissues was examined by RT-PCR, Western blot analysis, and immunohistochemistry. 63 endometrioid and 10 papillary serous types of fresh cases and 81 endometrioid and 7 papillary serous types of paraffin-embedded cases were studied. Regulation of clusterin expression by beta-estradiol in HEC-1B and HEC-1A cells was investigated using RT-PCR and Western blot analysis. Cell proliferative function of clusterin was examined in 293T cells.Results. We found higher expression of clusterin in endometrioid compared to papillary serous carcinoma using both immunohistochemistry (p=0.033) and Western blot analysis (p=0.024). The mRNA and protein expressions of clusterin in endometrioid carcinoma were higher than in benign endometrium (p=0.002). Forced expression of clusterin promoted 293T cell survival in a concentration-dependent manner, and estradiol treatment increased clusterin expression in HEC-1B but not in HEC-1A cells.Conclusions. These data suggest that clusterin expression is related to endometrioid carcinoma of endometrium, in which estrogen is involved in the regulatory network of clusterin. (c) 2008 Elsevier Inc. All rights reserved.