Resolution of three distinct populations of nerve endings from rat brain homogenates by zonal isopycnic centrifugation.
Resolution of three distinct populations of nerve endings from rat brain homogenates by zonal isopycnic centrifugation.
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DOI：
10.1083/jcb.61.2.466
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发表时间：
1974-05
期刊：
影响因子：
--
通讯作者：
Hodel C
中科院分区：
文献类型：
--
作者：
Bretz U;Baggiolini M;Hauser R;Hodel C
利用B-XIV转子在连续蔗糖密度梯度下建立了分区等差平衡分离大鼠前脑匀浆亚细胞组分的条件。对各组分进行生化分析和超微结构形态测定。从前脑匀浆的核后上代开始，已经有可能从彼此中分离出三种不同的神经末梢群体，以及从游离线粒体和髓磷脂碎片中分离出三种不同的神经末梢群体。三种类型的神经末梢在表观比重、生化特性和体外选择性积累外源性递质能力等方面存在差异。按模态平衡密度的递增顺序，这三个粒子群可能代表(a)胆碱能神经末梢，其特征是乙酰胆碱含量高，(b) γ-氨基丁酸（GABA）含神经末梢，具有高谷氨酸脱羧酶活性和积累外源GABA的能力，(c)肾上腺素能神经末梢，积累外源多巴胺和去甲肾上腺素，具有高单胺氧化酶活性。
Conditions have been established for the fractionation of subcellular components of rat forebrain homogenates by zonal isopycnic equilibration in continuous sucrose density gradients using a B-XIV rotor. The fractions were analyzed biochemically and by ultra-structural morphometry. Starting from postnuclear supernates of forebrain homogenates, it has been possible to resolve three distinct populations of nerve endings from one another, as well as from free mitochondria and myelin fragments. The three types of nerve endings differ in their apparent specific gravity, their biochemical properties, and their ability selectively to accumulate exogenous transmitter substances in vitro. These three particle populations are likely to represent, in order of increasing modal equilibrium density, (a) cholinergic nerve endings, characterized by their high content of acetylcholine, (b) γ-amino butyric acid (GABA)-containing nerve endings with high glutamate decarboxylase activity and the ability to accumulate exogenous GABA, (c) adrenergic nerve endings that accumulate exogenous dopamine and noradrenaline and exhibit high monoamine oxidase activity.