Curcumin as fluorescent probe for directly monitoring in vitro uptake of curcumin combined paclitaxel loaded PLA-TPGS nanoparticles
Curcumin as fluorescent probe for directly monitoring in vitro uptake of curcumin combined paclitaxel loaded PLA-TPGS nanoparticles
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DOI：
10.1088/2043-6262/7/2/025001
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发表时间：
2016-06-01
影响因子：
2.1
通讯作者：
My Nhung Hoang Thi
中科院分区：
文献类型：
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作者：
Hoai Nam Nguyen;Phuong Thu Ha;My Nhung Hoang Thi
治疗诊断学是一种将治疗和诊断能力结合在一起的方法，是临床应用和研究的一种有前途的工具。虽然有许多生色团可用于光学成像，但由于光漂白性质或固有毒性，它们的应用受到限制。姜黄素是从姜黄根茎中提取的一种天然化合物，由于其生物活性和强荧光性而被广泛认为是生物成像的生物相容性探针。在这项研究中，我们的目的是构建一个系统，具有双重功能：诊断和治疗，基于聚（丙交酯）-生育酚聚乙二醇琥珀酸酯（PLA-TPGS）胶束共负载姜黄素（Cur）和紫杉醇（PTX）。制备了两种姜黄素纳米粒，并用傅里叶变换红外光谱、场发射扫描电镜和动态光散射等方法对其进行了表征。姜黄素在这些系统中的细胞摄取和荧光活性也通过生物测定研究进行了测试，并与紫杉醇-奥美拉唑进行了比较。结果表明，（Cur + PTX）-PLA-TPGS纳米粒是一种潜在的癌症治疗诊断系统。
Theranostics, which is the combination of both therapeutic and diagnostic capacities in one dose, is a promising tool for both clinical application and research. Although there are many chromophores available for optical imaging, their applications are limited due to the photobleaching property or intrinsic toxicity. Curcumin, a natural compound extracted from the rhizome of curcuma longa, is well known thanks to its bio-pharmaceutical activities and strong fluorescence as biocompatible probe for bio-imaging. In this study, we aimed to fabricate a system with dual functions: diagnostic and therapeutic, based on poly(lactide)-tocopheryl polyethylene glycol succinate (PLA-TPGS) micelles co-loaded curcumin (Cur) and paclitaxel (PTX). Two kinds of curcumin nanoparticle (NP) were fabricated and characterized by Fourier transform infrared spectroscopy, field emission scanning electron microscopy and dynamic light scattering methods. The cellular uptake and fluorescent activities of curcumin in these systems were also tested by bioassay studies, and were compared with paclitaxe-oregon. The results showed that (Cur + PTX)-PLA-TPGS NPs is a potential system for cancer theranostics.