Evaluation of the Internal Structure of Normal and Pathological Guinea Pig Cochleae Using Optical Coherence Tomography
Evaluation of the Internal Structure of Normal and Pathological Guinea Pig Cochleae Using Optical Coherence Tomography
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DOI：
10.1159/000354620
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发表时间：
2013-01-01
影响因子：
1.6
通讯作者：
Yamasoba, Tatsuya
中科院分区：
文献类型：
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作者：
Kakigi, Akinobu;Takubo, Yuya;Yamasoba, Tatsuya
通过控制标本的切片轴并避免由于组织学制备过程中引起的伪影而造成的任何误解，我们的技术将有助于在没有或之前进行组织学评估的情况下检查耳蜗病理。光学相干断层扫描 (OCT) 使体内多个器官（例如眼睛）的内部结构可视化成为可能。尽管内耳内部结构的可视化已被用来尝试和识别某些病理状况，但尝试失败的主要原因是该末端器官周围有厚厚的骨囊。用乙二胺四乙酸对耳蜗骨壁进行脱钙后，我们可以通过 OCT 清晰地看到其内部结构。我们发现了内淋巴积水、纹状体萎缩和柯蒂氏器损伤，分别表现为赖斯纳膜扩张、侧壁变薄和柯蒂氏器变平。当石蜡包埋、切片并用苏木精和伊红 (HE) 染色的标本在光学显微镜下检查时，正常和病理耳蜗的 OCT 图像与 HE 标本的图像几乎相同，只是 HE 标本表现出一些在 OCT 图像中无法识别的伪影，这些伪影被认为是在制备过程中引起的。由于 OCT 使人们能够通过操纵标本的切片轴来获得任意平面图像，并避免由于组织学准备过程中引起的伪影而导致的任何误解，因此我们的技术将可用于在没有组织学评估或之前进行组织学评估的情况下检查耳蜗病理。 (C) 2013 S. Karger AG，巴塞尔
nipulating the slice axis of the specimens and avoids any misinterpretation due to artifacts induced during histological preparation, our technique would be useful for examining cochlear pathologies without or prior to histological evaluations. Optical coherence tomography (OCT) makes it possible to visualize the internal structures of several organs, such as the eye, in vivo. Although visualization of the internal structures of the inner ear has been used to try and identify certain pathological conditions, attempts have failed mainly due to the thick bony capsule surrounding this end organ. After decalcifying the bony wall of the cochlea with ethylenediamine tetraacetic acid, we could clearly visualize its internal structures by using OCT. We identified endolymphatic hydrops, strial atrophy and damage to the organ of Corti, evident as a distention of Reissner's membrane, thinning of the lateral wall and flattening of the organ of Corti, respectively. When specimens embedded in paraffin, sliced and stained with hematoxylin and eosin (HE) were examined under a light microscope, the OCT images of normal and pathological cochleae were virtually identical with those of the HE specimens, except that the HE specimens exhibited several artifacts unrecognized in OCT images, which were considered to be induced during the preparation process. Since OCT enables one to obtain arbitrary plane images by manipulating the slice axis of the specimens and avoids any misinterpretation due to artifacts induced during histological preparation, our technique would be useful for examining cochlear pathologies without or prior to histological evaluations. (C) 2013 S. Karger AG, Basel