IgA antibodies recognizing LABD97 are predominantly IgA1 subclass.
IgA antibodies recognizing LABD97 are predominantly IgA1 subclass.
复制标题
识别 LABD97 的 IgA 抗体主要是 IgA1 亚类。
DOI：
10.1080/000155599750010229
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发表时间：
1999
影响因子：
3.6
通讯作者：
Zone,JJ
中科院分区：
文献类型：
--
作者：
Egan,CA;Martineau,MR;Taylor,TB;Meyer,LJ;Petersen,MJ;Zone,JJ
线性IgA大疱性皮肤病是一种罕见的获得性表皮下皮肤起泡性疾病。线状IgA大疱性皮肤病中公认的抗原是一种被称为LABD97的97 kda基膜带蛋白。先前使用immunoŊuorescent技术的研究表明，IgA反应仅限于IgA1亚类。我们研究了6例含有与LABD97反应的循环IgA抗体的患者血清中的IgA抗体亚类。方法包括直接、间接immunoŊuorescence和免疫印迹法。3种方法均检测到IgA1抗labd97抗体。2例患者直接immunoŊuorescence检测IgA2抗体。3例患者间接immunoŊuorescence有IgA2抗体。其中2例也有抗labd97 IgA2抗体，1例在Western免疫印迹上有含有抗labd97 IgA抗体的分泌成分。我们得出结论，在LABD中与LABD97反应的主要IgA抗体亚类是IgA1，尽管在某些情况下可能涉及IgA2亚类。关键词：皮肤；基底膜;水泡。（1999年3月11日接受）journal of dermatology; 1999；79: 343ą346。CA Egan，犹他大学医学院皮肤科，50 North Medical Drive, Salt Lake City, UT 84132， USA电子邮件：CA Egan@ m.c c. utah。edu。线性IgA大疱性皮肤病（LABD）是一种罕见的皮肤获得性表皮下起疱性疾病，基于其独特的沿皮肤基底膜线性IgA的免疫病理发现而被定义(1)。LABD免疫电镜显示三种类型的IgA沉积：层下致密沉积；透明层沉积；以及两个地点的沉积（2ą5）。我们之前曾报道过一种从人表皮中提取的97-kDa抗原（LABD97），该抗原可与透明层型LABD患者血清中的IgA基底膜带（BMZ）抗体结合(6)。我们已经证明LABD97抗原与大疱性类天疱疮抗原BPAg2的细胞外结构域的一部分相同(7)。结合LABD97的IgA抗体通过免疫金电镜将抗原定位在BPAg2的NC16A和羧基末端结构域之间的透明层（8,9）。据免疫荧光技术报道，LABD中的BMZ抗体为IgA1亚型（10）。人血清中IgA的浓度约为IgG的五分之一，但它是分泌物中最常见的免疫球蛋白。它以2种同型形式存在，IgA1和IgA2，其结构差异约为20个氨基酸，主要位于铰链区（11）。在血清中，IgA1是主要亚类，而在分泌物中
Linear IgA bullous dermatosis is a rare acquired subepidermal blistering disease of the skin. A recognized antigen in linear IgA bullous dermatosis is a 97-kDa basement membrane zone protein termed LABD97. Previous studies, using immunoŊuorescent techniques, have suggested that the IgA response is restricted to the IgA1 subclass. We studied the IgA antibody subclasses in the sera of 6 patients that contained circulating IgA antibodies reactive with LABD97. The methods used included direct and indirect immunoŊuorescence and Western immunoblot. All patients tested had IgA1 anti-LABD97 antibodies detected by all 3 methods. Two patients had IgA2 antibodies detected by direct immunoŊuorescence. Three patients had IgA2 antibodies on indirect immunoŊuorescence. Two of these also had anti-LABD97 IgA2 antibodies and 1 had secretory component containing anti-LABD IgA antibodies on Western immunoblot. We conclude that the predominant IgA antibody subclass reactive with LABD97 in LABD is IgA1, although the IgA2 subclass may be involved in some cases. Key words: skin; basement membrane; blisters.(Accepted March 11, 1999.) Acta Derm Venereol 1999; 79: 343ą346. CA Egan, Department of Dermatology, University of Utah School of Medicine, 50 North Medical Drive, Salt Lake City, UT 84132, USA. E-mail: CA Egan@ m. cc. utah. edu.Linear IgA bullous dermatosis (LABD) is a rare acquired subepidermal blistering disease of the skin, which has been defined on the basis of its unique immunopathological finding of linear IgA along the cutaneous basement membrane (1). Immunoelectron microscopy in LABD reveals 3 patterns of IgA deposition: sub-lamina densa deposition; lamina lucida deposition; and deposition in both locations (2ą5). We have previously described a 97-kDa antigen (LABD97) extracted from human epidermis, which binds IgA basement membrane zone (BMZ) antibodies from sera of patients with the lamina lucida type of LABD (6). We have shown that this LABD97 antigen is identical to a portion of the extracellular domain of the bullous pemphigoid antigen, BPAg2 (7). The IgA antibodies that bind LABD97 localize antigens in the lamina lucida between the NC16A and carboxyl terminal domains of BPAg2 by immunogold electron microscopy (8, 9). It has been reported by immunofluorescent techniques that the BMZ antibodies in LABD are the IgA1 subtype (10). Human IgA is present in human serum at about one-fifth the concentration of IgG, but it is the most frequent immunoglobulin in secretions. It occurs in 2 isotypic forms, IgA1 and IgA2, which differ in structure by about 20 amino acids, predominantly located at the hinge region (11). In serum, IgA1 is the predominant subclass, while in secretions