Deficiency of heat shock transcription factor 1 suppresses heat stress-associated increase in slow soleus muscle mass of mice
Deficiency of heat shock transcription factor 1 suppresses heat stress-associated increase in slow soleus muscle mass of mice
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DOI：
10.1111/apha.12600
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发表时间：
2015-12-01
影响因子：
6.3
通讯作者：
Goto, K.
中科院分区：
文献类型：
--
作者：
Ohno, Y.;Egawa, T.;Goto, K.
目的研究热休克转录因子1（HSF 1）缺乏对小鼠比目鱼肌慢肌质量增加的影响。方法将HSF 1基因缺失的小鼠和野生型小鼠随机分为对照组和热应激组。热应激组小鼠暴露于热应激（41摄氏度，60分钟），在恒温箱中没有anesthesias.ResultsSignificant增加湿，干重，蛋白质含量的比目鱼肌在野生型小鼠7天后，观察应用的热应激。然而，热应激对HSF 1基因敲除小鼠的比目鱼肌质量没有影响。两种类型的小鼠表现出热应激对HSF mRNA表达的影响（HSF 1，HSF 2和HSF 4）。另一方面，热应激上调热休克蛋白（HSP）在mRNA（HSP 72）和蛋白质（HSP 72和HSP 110）水平的野生型小鼠，但没有在HSF 1-null小鼠。野生型小鼠比目鱼肌中Pax 7阳性细胞核相对于总肌核的数量在热应激下显著增加，但在HSF 1缺失小鼠中没有。此外，HSF 1基因的缺乏抑制热应激相关的磷酸化Akt和p70 S6激酶（p-p70 S6 K）在比目鱼肌muscle.ConclusionHeat stress-associated增加骨骼肌质量可能是由HSF 1和/或HSF 1介导的应激反应，激活肌肉卫星细胞和Akt/p70 S6 K信号通路。
AimEffects of heat shock transcription factor 1 (HSF1) deficiency on heat stress-associated increase in slow soleus muscle mass of mice were investigated.MethodsBoth HSF1-null and wild-type mice were randomly assigned to control and heat-stressed groups. Mice in heat-stressed group were exposed to heat stress (41 degrees C for 60min) in an incubator without anaesthesia.ResultsSignificant increase in wet and dry weights, and protein content of soleus muscle in wild-type mice was observed seven days after the application of the heat stress. However, heat stress had no impact on soleus muscle mass in HSF1-null mice. Neither type of mice exhibited much effect of heat stress on HSF mRNA expression (HSF1, HSF2 and HSF4). On the other hand, heat stress upregulated heat shock proteins (HSPs) at the mRNA (HSP72) and protein (HSP72 and HSP110) levels in wild-type mice, but not in HSF1-null mice. The population of Pax7-positive nuclei relative to total myonuclei of soleus muscle in wild-type mice was significantly increased by heat stress, but not in HSF1-null mice. Furthermore, the absence of HSF1 gene suppressed heat stress-associated phosphorylation of Akt and p70 S6 kinase (p-p70S6K) in soleus muscle.ConclusionHeat stress-associated increase in skeletal muscle mass may be induced by HSF1 and/or HSF1-mediated stress response that activates muscle satellite cells and Akt/p70S6K signalling pathway.