The methyl-CpG binding transcriptional repressor MeCP2 stably associates with nucleosomal DNA
The methyl-CpG binding transcriptional repressor MeCP2 stably associates with nucleosomal DNA
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DOI：
10.1021/bi990224y
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发表时间：
1999-06-01
期刊：
影响因子：
2.9
通讯作者：
Wolffe, AP
中科院分区：
文献类型：
--
作者：
Chandler, SP;Guschin, D;Wolffe, AP
我们研究了甲基- cpg结合转录抑制因子MeCP2与核小体DNA的相互作用。我们发现MeCP2通过甲基- cdg结合域（MBD）与暴露在主要凹槽中的甲基- cpgs相关的核小体DNA形成离散复合物。除了MBD外，MeCP2的羧基末端片段还有助于与裸DNA和核小体核心结合。这些观察结果提供了MeCP2进入染色质的分子机制，从而靶向进一步修饰染色质结构的辅抑制子复合物。
We have investigated the interactions of the methyl-CpG binding transcriptional repressor MeCP2 with nucleosomal DNA. We find that MeCP2 forms discrete complexes with nucleosomal DNA associating with methyl-CpGs exposed in the major groove via the methyl-CDG-binding domain (MBD). In addition to the MBD, the carboxyl-terminal segment of MeCP2 facilitates binding both to naked DNA and to the nucleosome core. These observations provide a molecular mechanism by which MeCP2 can gain access to chromatin in order to target corepressor complexes that further modify chromatin structure.