The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacle Balanus (=Amphibalanus) amphitrite
The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacle Balanus (=Amphibalanus) amphitrite
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DOI：
10.1242/jeb.070235
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发表时间：
2012-11
影响因子：
2.8
通讯作者：
Yu Zhang;Li-Sheng He;Gen Zhang;Ying Xu;O. Lee;K. Matsumura;P. Qian
中科院分区：
文献类型：
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作者：
Yu Zhang;Li-Sheng He;Gen Zhang;Ying Xu;O. Lee;K. Matsumura;P. Qian
藤壶藤壶是热带和亚热带地区潮间带岩石海岸上最主要的污损生物之一，因此是海洋生物研究的目标生物。从成虫释放出来后，游泳的无节幼体进行六个蜕皮周期，然后转化为一个塞浦路斯。利用成对的触角，一个有能力的塞浦路斯积极探索和选择一个合适的附着和变态（统称为定居）的基质。这一选择过程涉及外源信号的接收和随后的内源信号转导。探讨一氧化氮（NO）和环鸟苷酸（cGMP）在B幼虫附着过程中的作用。amphitrite，我们研究了一个NO供体和一个NO清除剂，两个一氧化氮合酶（NOS）抑制剂和可溶性鸟苷酸环化酶（sGC）抑制剂对沉降的塞浦路斯。我们发现NO供体硝普钠（SNP）以剂量依赖的方式抑制幼虫沉降。相反，NO清除剂羧基PTIO和NOS抑制剂氨基胍半硫酸盐（AGH）和S-甲基异硫脲硫酸盐（SMIS）显着加速幼虫的定居。抑制下游鸟苷酸环化酶（GC）活性使用GC选择性抑制剂ODQ也可以显着加速幼虫定居。有趣的是，在所有测试浓度下，SNP的沉降抑制作用可以被ODQ减弱。在NOS和sGC的发育表达谱中，这两个基因的最低表达在Cyprid阶段被检测到，这是幼虫决定附着和变形的关键阶段。综上所述，我们得出结论，NO调节幼虫定居通过介导下游cGMP信号。
SUMMARY The barnacle Balanus amphitrite is among the most dominant fouling species on intertidal rocky shores in tropical and subtropical areas and is thus a target organism in antifouling research. After being released from adults, the swimming nauplius undertakes six molting cycles and then transforms into a cyprid. Using paired antennules, a competent cyprid actively explores and selects a suitable substratum for attachment and metamorphosis (collectively known as settlement). This selection process involves the reception of exogenous signals and subsequent endogenous signal transduction. To investigate the involvement of nitric oxide (NO) and cyclic GMP (cGMP) during larval settlement of B. amphitrite, we examined the effects of an NO donor and an NO scavenger, two nitric oxide synthase (NOS) inhibitors and a soluble guanylyl cyclase (sGC) inhibitor on settling cyprids. We found that the NO donor sodium nitroprusside (SNP) inhibited larval settlement in a dose-dependent manner. In contrast, both the NO scavenger carboxy-PTIO and the NOS inhibitors aminoguanidine hemisulfate (AGH) and S-methylisothiourea sulfate (SMIS) significantly accelerated larval settlement. Suppression of the downstream guanylyl cyclase (GC) activity using a GC-selective inhibitor ODQ could also significantly accelerate larval settlement. Interestingly, the settlement inhibition effects of SNP could be attenuated by ODQ at all concentrations tested. In the developmental expression profiling of NOS and sGC, the lowest expression of both genes was detected in the cyprid stage, a crucial stage for the larval decision to attach and metamorphose. In summary, we concluded that NO regulates larval settlement via mediating downstream cGMP signaling.