Cell specific dopamine modulation of the transient potassium current in the pyloric network by the canonical D1 receptor signal transduction cascade.
Cell specific dopamine modulation of the transient potassium current in the pyloric network by the canonical D1 receptor signal transduction cascade.
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细胞特异性多巴胺通过经典 D1 受体信号转导级联调节幽门网络中的瞬时钾电流。
DOI：
10.1152/jn.00195.2010
复制
发表时间：
2010
影响因子：
2.5
通讯作者：
Baro,DeborahJ
中科院分区：
文献类型：
--
作者：
Zhang,Hongmei;Rodgers,EdmundW;Krenz,Wulf-DieterC;Clark,MerryC;Baro,DeborahJ
多巴胺（DA）通过直接作用于每一个回路神经元来改变多刺龙虾口胃神经节（STG）中幽门网络产生的运动模式。14个幽门神经元分为6种细胞类型，DA的作用是细胞类型特异性的。Shal通道（IA）介导的瞬时钾电流是大多数细胞类型中DA调节的共同靶点。DA使IA的电压依赖性在幽门扩张器（PD）和幽门外侧（LP）神经元中向相反的方向移动。IA的细胞类型特异性DA调节的机制尚不清楚。DA受体（DAR）可分为1型（D1 R）和2型（D2 R）。已知D1 R和D2 R分别增加和降低细胞内cAMP浓度。我们假设DA对PD和LPIA的相反作用是由于DAR表达模式的差异。在本研究中，我们发现，LP表达体树突D1 Rs集中在突触附近，但不表达D2 Rs。DA对LPIA的调节是通过Gs-adenylyl cyclase-cAMP-protein kinase A途径介导的。此外，我们定义了龙虾D1 Rs（氟噻吨）和D2 Rs（甲氧氯普胺）在异源表达系统中的拮抗剂，并表明DA对LPIA的调节被氟噻吨阻断，但不被甲氧氯普胺阻断。我们以前的研究表明，PD神经元表达D2 Rs，但不表达D1 Rs，从而支持了DA对IA的细胞特异性作用是由于受体表达差异的观点。
Dopamine (DA) modifies the motor pattern generated by the pyloric network in the stomatogastric ganglion (STG) of the spiny lobster, Panulirus interruptus, by directly acting on each of the circuit neurons. The 14 pyloric neurons fall into six cell types, and DA actions are cell type specific. The transient potassium current mediated by shal channels (IA) is a common target of DA modulation in most cell types. DA shifts the voltage dependence ofIAin opposing directions in pyloric dilator (PD) versus lateral pyloric (LP) neurons. The mechanism(s) underpinning cell-type specific DA modulation ofIAis unknown. DA receptors (DARs) can be classified as type 1 (D1R) or type 2 (D2R). D1Rs and D2Rs are known to increase and decrease intracellular cAMP concentrations, respectively. We hypothesized that the opposing DA effects on PD and LPIAwere due to differences in DAR expression patterns. In the present study, we found that LP expressed somatodendritic D1Rs that were concentrated near synapses but did not express D2Rs. Consistently, DA modulation of LPIAwas mediated by a Gs-adenylyl cyclase-cAMP-protein kinase A pathway. Additionally, we defined antagonists for lobster D1Rs (flupenthixol) and D2Rs (metoclopramide) in a heterologous expression system and showed that DA modulation of LPIAwas blocked by flupenthixol but not by metoclopramide. We previously showed that PD neurons express D2Rs, but not D1Rs, thus supporting the idea that cell specific effects of DA onIAare due to differences in receptor expression.
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DOI：
10.1016/s0021-9258(19)34116-x
发表时间：
1990-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Chijiwa;A. Mishima;M. Hagiwara;M. Sano;Kyozo Hayashi;Tsutomu Inoue;Naito Kenji;T. Toshioka
通讯作者：
T. Chijiwa;A. Mishima;M. Hagiwara;M. Sano;Kyozo Hayashi;Tsutomu Inoue;Naito Kenji;T. Toshioka
影响因子：
2.5
作者：
Cleland, TA;Selverston, AI
通讯作者：
Selverston, AI
影响因子：
2.5
作者：
通讯作者：
--
DOI：
10.1016/j.cbpb.2006.08.018
发表时间：
2007-01-01
影响因子：
2.2
作者：
Clark, Merry C.;Baro, Deborah J.
通讯作者：
Baro, Deborah J.
腺苷酸环化酶催化核心的结构
影响因子：
64.8
作者：
Gongyi Zhang;Yu Liu;A. Ruoho;J. Hurley
通讯作者：
J. Hurley