Effects of oocyte vitrification on gene expression in the liver and kidney tissues of adult offspring
Effects of oocyte vitrification on gene expression in the liver and kidney tissues of adult offspring
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DOI：
10.1007/s10815-022-02611-z
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发表时间：
2022-10-12
影响因子：
3.1
通讯作者：
Zhang，Cuilian
中科院分区：
文献类型：
--
作者：
Zhang，Lei;Chen，Huanhuan;Zhang，Cuilian
卵母细胞玻璃化是一项重要的辅助生殖技术，它保留了未婚恶性肿瘤患者的生育能力，促进了卵母细胞捐赠计划的发展。近年来，抗逆转录病毒技术对后代健康的影响，包括卵母细胞和胚胎的玻璃化，引起了人们的广泛关注；然而，很难在人体中进行长期随访和生化评价。在本研究中，我们首次通过RNA测序检测了卵母细胞玻璃化对成年小鼠后代器官中基因表达的影响。我们的结果显示，只有少量基因表达受到显著影响。7个基因（Tpm3、Hspe1-rs1、Ntrk2、Cyp4a31、Asic5、Cyp4a14、Retsat）在肝脏中异常表达，10个基因（Lbp、Hspe1-rs1、Prxl2b、Pfn3、Gm9008、Bglap3、Col8a1、Hmgcr、Ero1lb、ifi441）在肾脏中异常表达。一些基因与肝脏或肾脏的代谢和疾病发生有关。此外，我们还特别关注了已知印迹基因和DNA甲基化相关基因在成体器官中的表达，这些基因在着床前阶段易受卵母细胞低温保存的影响。结果，在成人器官中检测到一些转录本，但它们不受卵母细胞玻璃化的影响。总之，我们首次报道了卵母细胞玻璃化没有显著改变后代器官的整体基因表达；尽管如此，它仍然可以影响一些功能基因的转录。卵母细胞玻璃化可能引起的不良反应值得关注和进一步研究。
Oocyte vitrification is an important assisted reproductive technology (ART) that preserves the fertility of unmarried patients with malignant tumors, and promotes the development of the oocyte donation program. In recent years, the effects of ART, including the vitrification of oocytes and embryos on the health of offspring, have attracted much attention; however, it is difficult to conduct long-term follow-up and biochemical evaluation in humans. In this study, we detected the effect of oocyte vitrification on gene expression in the organs of adult mice offspring by RNA sequencing for the first time. Our results showed that only a small amount of gene expression was significantly affected. Seven genes (Tpm3, Hspe1-rs1, Ntrk2, Cyp4a31, Asic5, Cyp4a14, Retsat) were abnormally expressed in the liver, and ten genes (Lbp, Hspe1-rs1, Prxl2b, Pfn3, Gm9008, Bglap3, Col8a1, Hmgcr, Ero1lb, Ifi44l) were abnormal in the kidney. Several genes were related to metabolism and disease occurrence in the liver or kidney. Besides, we paid special attention to the expression of known imprinted genes and DNA methylation–related genes in adult organs, which are susceptible to oocyte cryopreservation in the preimplantation stage. As a result, some of these transcripts were detected in adult organs, but they were not affected by oocyte vitrification. In conclusion, we first report that oocyte vitrification did not significantly change the global gene expression in offspring organs; nonetheless, it can still influence the transcription of a few functional genes. The potential adverse effects caused by oocyte vitrification need attention and further study.